Hibiscus pollens

I got my foldscope a few weeks ago and I spent many days learning how to use it. My cousin, who is doing bio-tech , helped me a lot in preparing slides and taking photos.

Following are the pictures of pollen grains of hibiscus plant in our garden taken with Moto G (5 mega pixel camera) through 140x lens. I couldn’t show you images through 400x because I am still not good with it.

pollen 1 pollen 2 polen 3

I would like you , (the viewers) to suggest me experiments related to this , if any. I would definitely try them out.

~Niramay Gogate

 

 

 

7 Comments Add yours

  1. laksiyer says:

    Great start. Suspend them in water/ glycerol and see if they undergo a size change. See if they are visible in their anthers before bursting and study their packing. 400x would be great for surface features for such large pollen. Note that if you are using a coversiip in 400x, its thickness should be below 0.17 mm ,else use tape.

  2. Niramay Gogate says:

    @laksiyer, Thank you for your suggestions. I will try those things and inform you about them as soon as I finish all of them

  3. Manu Prakash says:

    @Niramay: I am thrilled the microscopes finally reached India 🙂

    M

  4. Niramay Gogate says:

    @laksiyer, It took me a bit long to find glycerol. When I suspended them in glycerol; I got almost the same results as I suspended them in water. I think it is somewhat related to osmosis.
    I tried 400x lens but didn’t get good results because the light coming was too dim. I will have to deal with 400x more carefully. I used ordinary tape for 400x.
    Luckily I have two more species of hibiscus in my garden. So I am planning to compare pollens of all of them ; but it will take time since it is raining in our city these days.

  5. Manu Prakash says:

    @Niramay/@Laksiyer: I went to a friends wedding last night and swept a lot of flowers 🙂

    I will be starting experiments in trying to germinate the pollen grains today. The plan is to do time lapse imaging of the pollen tube. It’s presumably fastest growing cell known – so I am excited for what I find.

    Will share my protocols; as I get them to work.

    Cheers
    Manu

  6. laksiyer says:

    @Niramay: Looking forward to the pics. @Manu: Germinating pollen grains sounds like fun. I know sucrose is a big component but have never tried it.

  7. Niramay Gogate says:

    @laksiyer &@Manu, I am sorry for late reply. I was in a small village in Germany last two weeks.so I could not check the website.

    I will try to upload further pics soon

    Recently, I found two more species of hibiscus. So I wish to compare their pollens with previous one

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