What does a 20cents razor, food coloring and plant stems have in common?

That sounds like a silly question – and it is. But sometime silly things are good. In this post; I will describe how to use a “disposable shaving razor” and some food coloring to dive into the inner world of plant plumbing. You heard it right – plumbing. 

Plants (and trees) are the worlds most advance microfluidics system. Microfluidics is just a fancy word for plumbing at small scale – actually at a scale so small that all the plumbing tubes and pipes are made of individual cells – and thus range roughly the size of single cells (10 or 100 micron). But trees are known to make microfluidic channels that can pump fluids tens of meters high. And since we live in north California; I see redwood trees – which have been found to pump water more than 100 meters in height. That’s an incredible feat; specially when you think about doing this through pipes that are no thicker than a slice of your hair thickness. 

But before we get too excited about figuring out how the hell does something like this work – we need to learn the basics of being able to watch how plants actually perform this  task. Here we will learn how to precisely slice up plant stems to see inside this hidden world. 

Here are some items you need: 
1. A disposable razor – costs 20 cents. 
http://mobile.walmart.com/ip/Schick-Twin-Blade-Plus-Disposable-Razors-12ct/20713694

Caution: razors are sharp; which means they can cut you!!! PLEASE PERFORM THESE STEPS IN SUPERVISION OF AN ADULT. 

2. Any Food coloring to color the cell wall of the stem. 
http://www.amazon.com/b?node=13825861

3. Any plants you want to look at. 

Methods: 
1. First, we will break open the disposable razor to get the two blades out. Please handle the blades very very carefully; and dispose them properly after use!!! One razor should give you two blades to work with. Since We want to perform transmission light microscopy; we need to cut the sample thin enough such that light can pass through. With 10 min of practice; you can get quiet good. I think I was regularly slicing 100 micron slices. It’s quiet remarkable to think that mane commercial sectioning machines can cut tens of nanometer in Slice thickness. But our slices are good enough for what we want to do. 

   
   2. Gently put the slices on a glass slide. I added a little bit of sugar water to just keep them moist. 

3. Put a drop of food coloring if you want to capture high contrast images of the cell wall. You don’t need to color the same; it’s just fun to see how contrast images. 

4. Mount your samples (with or without coverslip in your Foldscope and collect data. 
  

Here are the images and data I collected; for three different flowering plants. These are just representative images.. 

   
       
By leaving the slides in food colored droplets for a little bit of time (2 min); I was able to label the cell walls differential. Here are the results. 

    
                 

Next post, I will describe the various characteristic parts we can notice in the stem. Until that time; 

Keep Exploring. 

Cheers

Manu 

4 Comments Add yours

  1. laksiyer says:

    Hi Manu.. This is just simply wonderful. I need to try this as razor blades are in plenty here. Cant wait to read about your explorations on transport. I know of one other elegant technique that I read of but havent tried, where the two blades are put together. http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artapr04/wdslicera.html

  2. Manu Prakash says:

    That’s a wonderful pointer @Laksiyer. The double blade is a very neat idea – and as you see – disposable razors do come with two blades 🙂

    I will be testing this double razor trick tonight and report back. My idea is to actually build a 3D stack of the sections – to do simple tomography using Foldscope. Some of the image detection algorithms have gotten very good now – so I want to see how different xylem and phloem sections merge.

    Thanks for this pointer.

    Cheers
    Manu

  3. laksiyer says:

    That would be super Manu– a tomography. Please keep us posted about the sofrware that you are using too.. Hopefully some of them are freeware.

  4. Kate Honan says:

    Thank you for this great technique! Can’t wait to use the safer steps with students (I’ll do the cutting).

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