Polyplastron in rumen fluid

DBT Foldscope scheme –“Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Rumen fluid was collected from a healthy cow. Smear was prepared with one drop of rumen fluid, stained with lugol’s iodine and visualized under foldscope. Protozoa under the genus Polyplastron was identified.

Entodinium nanellum in rumen fluid

DBT scheme –“Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Protozoa under the genus Entodinium Entodinium nanellum was identified using foldscope. Morphology of Entodinium nanellum is ovoid, found in the rumen of cattle.

Diplodinium monocanthum in rumen fluid

DBT scheme –“Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Protozoa under the genus Diplodinium monocanthum Diplodinium monocanthum was identified using foldscope. Morphology of Diplodinium monocanthum is oval to triangular with posterioroblique and truncated end. It is found in the rumen of cattle.

Ostrachodinium tiete in rumen fluid

DBT scheme –“Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Protozoa under the genus Ostrachodinium Ostrachodinium tiete was identified using foldscope. Morphology of Ostrachodinium tiete is ellipsoid, with small lobe at the posterior side.

Metadinium in rumen fluid

DBT Foldscope scheme – “Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Rumen fluid was collected from a healthy cow. Smear was prepared with one drop of rumen fluid, stained with lugol’s iodine and visualized under foldscope. Protozoa under the genus Metadinium was identified based on the morphology- two skeletal plates fused…

Dasytricha in Rumen fluid

DBT Foldscope scheme – “Foldscope for dignosis of rumen acidosis and parasitic infection in cattle” Rumen fluid was collected from a healthy cow. Smear was prepared with one drop of rumen fluid, stained with lugol’s iodine stain and visualized under foldscope. Protozoa under the genus Dasytricha was identifid based on the morphology -Oval body, covered…

Entodinium yunnense in rumen fluid

DBT Foldscope scheme –“Foldscope for dignosis of rumen acidosis and parasitic infectionin cattle”. Protozoa under the genus Entodinium Entodinium yunnense was identified using Foldscope. Morphology of Entodinium yunnense is ellipsoidal and it is found in the rumen of cattle.

Enoploplastron in rumen fluid

DBT Foldscope scheme –“Foldscope for diagnosis of rumen acidosis and parasitic infection in cattle”. Protozoa present in rumen fluid under the genus Enoploplastron was identified using Foldscope. Enoploplastron is elongated, slightly ellipsoidal in shape.

Epidinium in rumen fluid

DBT Foldscope scheme- “Foldscope for dignosis of rumen acidosis and parasitic infection in cattle” Epidinium is a rumen ciliate, present in large numbers in cattle fed with high starch diets.

Eudiplodinium in rumen fluid

DBT Foldscope scheme- “Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Rumen Protozoa under the genus Eudiplodinium was identified using foldscope.

Ostrachodinium in rumen fluid

DBT Foldscope scheme –” Foldscope for dignosis of rumen acidosis and parasitic infection in cattle”. Rumen Protozoa under the genus Ostrachodinium was identified using Foldscope. Ostrachodinium is having one large skeletal plate of board shaped and three contractile vacuoles between macronucleus and left body wall. Body ends with large lobe. It is found in the…

Diplodinium in rumen fluid

DBT Foldscope scheme – “Foldscope for diagnosis of rumen acidosis and parasitic infection in cattle” Rumen fluid was collected from a healthy cow. Smear was prepared with one drop of rumen fluid, stained with methyl green and visualized under foldscope. Protozoa under the genus Diplodinium was identified. Morphology of Diplodinium is large rounded body, thick…

DBT twinning programme on Foldscope project with North Eastern Institute

DBT Foldscope scheme- “Foldscope for diagnosis of rumen acidosis and parasitic infections in cattle” DBT Twinning programme on foldscope project with north eastern institute Foldscope project functioning at the Department of Animal biotechnology has been twinned with Department of Biotechnology, Donbosco college, Maram, Manipur. Mr. S. Rajkumar, Head of the Department, Department of Biotechnology, Donbosco…

Spirochetes in rumen fluid

DBT Foldscope scheme – “Foldscope for diagnosis of rumen acidosis and parasitic infections in cattle” Spirochetes are motile, spiral bacteria. Bovine rumen fluid contains large numbers of spirochetes. Spirochetes are capable of fermenting polymers that are commonly present in the plant materials. Polymers such as xylan, pectin and arabinogalactan are used as substrate by spirochetes….

Holotrich Protozoa identification in a field setting

DBT Foldscope scheme – “Foldscope for diagnosis of Rumen acidosis and Parasitic infection in cattle”. This post is in response to Manu Prakash’s comment on our previous post (Holotrich protozoa – from Rumen fluid). Rumen fluid was collected from an healthy cow  (Figure 1) in a sterile container (figure 2).  A drop of rumen fluid was…

Hyalomma Tick viewed under foldscope

Ticks were collected from cattle and stored in 10% Formalin. Based on the morphology, the tick was identified as Hyalomma under foldscope. These tick are longirostrate ( long mouth parts) (figure1). It was observed that the 1st Coxa is deeply bifid ( figure 2).

Helminth Eggs

Helminths are large multi-cellular permanent parasitic worms, which can be seen with  our naked eyes. Knowledge of various species of helminth affecting the cattle will give an idea about the nature and intensity of helminthosis in bovine. They live and feed on living hosts. Helminths include Trematodes, cestodes, and nematodes.Trematodes are commonly called as flat…

Boophilus microplus (male tick)

Ticks were collected from cattle and stored in 10% Formalin. Based on the morphology, the tick was identified as Boophilus microplus (male) under foldscope. It was observed that Boophilus microplus are brevirostrates(have short mouth parts). A dorsal hump and ventral notch was also visible in the 1st Coxa. The presence of caudal process (in male…

Rhipicephalus sanguineus

Ticks were collected from dogs and stored in 10% Formalin. Based on the Anatomy, the tick was identified as Rhipicephalus sanguineus under foldscope.

Haemonchus contortus

Haemonchus contortus – These blood suckers are otherwise known as Stomach worm, Barber’s pole worm, is highly economically important Strongyle of sheep and goat. The anterior ends of both males and females are similar ( figure 1). The posterior end of female, the vulva is covered by a cuticular flap ( vulval flap or vulval knob)…

Larval stages of Filarial nematodes

One milliliter of blood was mixed with nine milliliter of 2% formalin. The mixture was centrifuged at 5,000 rpm for 5 min and the supernatant was discarded. To the sediment, few drops of methylene blue solution was added. A drop of the solution was placed on slide with a cover slip above and examined under…

Amphistome egg

2-3 g of dung sample was taken, mixed with water and then sieved. The mixture was centrifuged at 2000 rpm for about 2 minutes. The supernatant was discarded and a drop of the sediment was placed on a slide with a cover clip on top. It was then examined under foldscope. It was observed that…

Rumen protozoa stained with Lugol’s Iodine

DBT Foldscope scheme – “Foldscope for diagnosis of Rumen acidosis and Parasitic infections in cattle.” Rumen fluid was collected from an healthy cow and stained with Lugol’s iodine, a dye mainly used to stain and visualize the skeletal plates in protozoa.

Posterior Spircale of Chrysomia species

DBT Foldscope scheme “Foldscope for diagnosis of rumen acidosis and parasitic infections in cattle” Here’s a beautiful and detailed picture of the processed Posterior Spiracle of a Chrysomya larvae which was collected from an infected wound of a cow. The steps involved are: The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly…

One Day Workshop on Foldscope- It’s assembly and applications. Conducted by Madras Veterinary College

DBT Foldscope scheme – ” Foldscopes for diagnosis of rumen acidosis and parasitic infections in cattle”. A one Day Workshop on Foldscope’- It’s assembly and applications was conducted on 14.11.2018 at Student day care centre- seminar hall of Madras Veterinary college by the Dept. of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College Chennai – 07….

Foldscope Awareness program in S.J.T Surana Jain Vidyalaya School

DBT Foldscope scheme – “Foldscopes  for diagnosis of rumen acidosis and parasitic infections in cattle”.  An Awareness program on’ Introduction to Foldscope- An Origami paper microscope”  was held at S.J.T. Surana Jain Vidyalaya Higher Secondary School  ( No.2, Badrian Garden Lane, Park Town) Chennai – 600 003 for the class 9 students on 31.10.2018 by the Dept…

Isotricha Protozoa (from Rumen Fluid)

DBT Foldscope Scheme – “Foldscopes for diagnosis of rumen acidosis and parasitic infections in  cattle”. Rumen fluid from an healthy cow was taken, and stained with MFS stain  ( Methyl green Formaline Saline). This stain is mainly used to visualize the nucleus apparatus of the protozoa and  protozoa was identified as Isotricha  ( commonly present in…

Entodinium Protozoa ( from Rumen Fluid)

DBT Foldscope Scheme – “Foldscopes for diagnosis of rumen acidosis and parasitic infections in catte”. Rumen fluid from an healthy cow was taken, and stained with MFS stain  ( Methyl green Formaline Saline). Entodinium species was identified under Foldscope.  We were able to identify the various parts of the protozoa  ( Figure 1) Figure 1:…

Posterior Spircale of Stomoxys species

Stomoxys commonly known as stable fly resembles the house fly (Musca domestica).  Stable flies are obligate blood feeders and primarily attack cattle and horses for a blood meal (Entnemdept.ufl.edu, 2018) The Stomoxys larvae was collected from an infected wound and was processed. The steps involved are: The larvae was boiled in 10% NaOH for about 5…