Larval stages of Filarial nematodes

One milliliter of blood was mixed with nine milliliter of 2% formalin. The mixture was centrifuged at 5,000 rpm for 5 min and the supernatant was discarded. To the sediment, few drops of methylene blue solution was added. A drop of the solution was placed on slide with a cover slip above and examined under foldscope.

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