Two animals, a fungus and a houseplant — Part II The fungus

Continuing from my previous post, we now move on to the fungus associated with the scale insect. For this, I just cut a piece of the leaf with the black sooty material and put it on a slide and taped it with a cellophane tape.Unlike the scale insect views, which were the best I have had so far, the views of the fungus at 140x under the foldscope were  a bit disappointing. I could see fungal hyphae and conidia, and it confirmed that this black sooty stuff was the fungus. However, in the absence of nice stains like lactophenol cotton blue and poor expertise with fungal morphological forms I had to make do with what I saw. Note, below microscopic images were taken with a Foldscope fitted with a 140x lens and viewed with a Google nexus 5 phone.

fungus4
Unfortunately, I had broken my high-power foldscape lens holder and while tinkering with the lens, it popped out and into the unknown (Its a sore story), and hence I couldnt get a good high power foldscope image. So with some reluctance, I put the slide under my compound microscope at 400x. Taking pictures with my Compound Microscope is not as smooth as with the foldscope, but here is a collage of what I saw (Note below picture taken with a Celestron compound microscope 40x objective, 10x eyepiece and filmed with my google nexus 5 phone).

New folder4 The higher magnification was definitely more revealing. Studies on fungi that feed on the scale insect honeydew have shown that they belong to the Dothideomycete class of fungi, and the second picture on the top shows pigmented conidia just as in Aureobasidium.  The first picture on the left looks like Alternaria, but I am not too sure. One thing that became clear is that there is more than one type of fungus in this. These fungi necessarily need the honeydew to prosper and this can be seen in the fungal mats, which  are only present on the honeydew.

I also need to think about how I can display such fungi so that it is more useful for classification. More experiments are needed here and any recommendations are welcome. Finally, I had a more practical problem of saving my plant, and so I gave it a nice shower to reduce the load of scale insects and wash off the fungus.

More on the other animal in the party in my next post.. to be continued.

5 Comments Add yours

  1. Manu Prakash says:

    I know that a new kit is on it’s way to you. I put it in post last week 🙂

    I am so fascinated by this ecosystem inside an ecosystem inside an ecosystem..

    cheers
    manu

    1. laksiyer says:

      Thanks Manu. I hope to not disappoint. Foldscope has really changed my inertia towards putting things under a microscope and makes me think harder about how to design the next investigation.

  2. Niramay Gogate says:

    Oh guys, I haven’t even got my first foldscope! 🙁
    any way , this article was nice. I liked the way you explained everything

  3. Manu Prakash says:

    @Laksiyer: It’s really about lowering that inertia. This is what makes me most excited – we want people to do microscopy on dinner table, in the train, while commuting in the bus, in parks.. just about everywhere, all the time.

    @Niramay: Your kit is on it’s way. I personally posted it.

    manu

  4. Niramay Gogate says:

    @Manu- Thank you very much!
    It now seems that I will be joining your foldscope party very soon 🙂

Leave a Reply