Detecting Hepatitis B virus associated cellular changes in needle aspirate samples of HBV linked Hepatocellular carcinoma patients of different stages

Hematoxylin and Eosin stained sections from Fine Needle Aspirates (FNAC) samples of HCC patients

The photomicrgraphs taken with foldscope demonstrates higher stages of HCC patients . Figure a represents cancer cells in FNAC samples of a HCC stages patients which shows cellular clusters of small cells along with widened trabecular, multilayered cells and disorganized architecture. b, c and d are zoom out (b-2X, c-3X and d-4X) images of Fig a.

2 Comments Add yours

  1. SkyFromFoldscope says:

    Do you have a more detailed procedure for how you obtained, examined, and drew conclusions from the samples? This is very interesting.

    The Foldscope Team

    1. Ratna Kumari says:

      Hi Skylar

      We have posted 140X and its zoomed out pics. It is a needle aspirate samples of HCC patient the image shows widened trabecular pattern ( a deviation from normal hepatic architecture containing hexagonal hepatic lobules and radiating hepatic chords from central vein). It also demonstrates small cluster of cells and overlapped cells (a common feature fine needle cellular aspirates on slides). The sinusoids are dilated with lots of space between chord of hepatocytes. These features confirm higher stage of HCC patient. The first pathological examination was done by cytopathologist in blinded manner. As it is difficult to get FNAC samples from normal liver (Ethical concerns); so we do not have a comparative picture of normal liver fine needle cellular aspirates (which would show normal liver architecture with hexagonal lobules, small central vein, radiating hepatic chords, and thin sinusoids). But in our earlier posts we have posted the pics of chemical induced rat hepatocarcinogenesis model; demonstrating HCC features and normal liver features for comparative analysis.

      We will be posting some more pics of HCC FNAC samples.

      Let me know if you have any other question.

      Thank you

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