Skeleton Shrimp (Caprella) – day 2 of Hopkins course

Today i managed to put it in a skeleton shrimp in my foldscope.

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Here’s a video showing blood flowing through the shrimp. It’s incredible. You can see individual hemocytes speeding along.

I took a longer video with H, who studies these Caprella as a model organism, and she explained different features to us, including spotting a beating heart! I have to share a disclaimer that this particular shrimp is a female and we actually anesthetized, to extract the embryos, but looked like it wasn’t going to make it, and you’ll notice its heart beat is extremely low – i counted 2 beats separated by 8 seconds or so..

 

For those interested, here is a whole dissection video, but this was on a stereo microscope showing how we extracted the embryos for staining.

 

Finally, the class group is posting more pictures/videos live on to a class tumblr right here, in case you want to follow. I will share my foldscope videos and posts there, while simultaneously post long posts here directly on microcosmos too.

Link: http://hopkinsembryology2016.tumblr.com

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4 Comments Add yours

  1. laksiyer says:

    Speactacular. @Saad, are you using that PVC sheet slide? also could you make your picture clickable to expand. I wonder if this can be done throughout the website?

    1. Saad Bhamla says:

      @laks – the slide mount was just the old clay spacer technique. I don’t know how to make it clickable – the WordPress app doesn’t show me any options. Do you have any idea??

  2. laksiyer says:

    I guess you figured it out. When you edit the picture, you click link to media file. Now, it appears that if you do it once, that becomes the default for future posts. I wonder if it can be made a default for the all posts as often one wants to examine a picture more carefully. I tried using silly putty with wet samples, but mine just disintegrates and gets into the sample. IMust try some clay instead. I am also going to try PVC sheets and the plastic coverslip.

  3. Manu Prakash says:

    Beautiful video @Saad. What’s even more interesting about the cells in the hemolymph is that they don’t have too much of a guiding structure (like capillaries); but still the traffic control is beautiful.

    Keep exploring.

    cheers
    manu

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