Bacteria observed under Foldscope 000283BB6B01

Bacillus sp

Bacillus sp is isolated from the drinking water sample. It produced endospores that have the ability to resist heat, chemical treatment and radiation. Bacilli are ubiquitous, they can survive in the environment for many years due to the formation of spores. It produced central, terminal and lateral endospore.

MICROSCOPIC IMAGE FOLDSCOPE IMAGE

2 Comments Add yours

  1. Manu Prakash says:

    Beautiful images @Piruthika. Can you also share brief protocols for how the samples were collected and stained.

    You might also enjoy some of the live bacterial imaging on the site – one example here: https://microcosmos.foldscope.com/?p=16549

    Cheers
    Manu

    1. R.Piruthika says:

      Hello sir,
      Sorry for the delay
      Drinking water samples were collected from rural areas around Coimbatore. Aseptically transferred the samples to the laboratory. Serially dilute the samples and inoculate the dilution on the nutrient agar and incubate the plates. Then pick up a isolated colony and emulsify the colony using saline/distilled water on the glass slide and allowed to air dry and heat fixed. Then, the Gram staining technique was performed. The smear was flooded with primary stain (crystal violet) and let stand for about one minute and rinsed with tap water. The Gram’s iodine was added and let stand for one minute and rinsed with tap water. Then, decolonization (50% Acetone) step was followed. Finally, the counter-stain (Safranin) was added and let stand for 60 seconds and rinsed with water. The smear was air dried and viewed the smear by using Foldscope (140X) and Microscope (1000X).

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