The spores on mango kernels are stained with lactophenol cotton blue solution and observed under foldscope. Lactophenol cotton blue stain is formulated with lactophenol, which serves as a mounting fluid, and cotton blue. Organisms suspended in the stain are killed due to the presence of phenol. Cotton blue is an acid dye that stains the chitin present in the cell walls of fungi.
Fig. Fungal infested mango kernels
Fig. Infested fungus observed under Foldscope using Lactophenol cotton blue stain