The Great Blue Whale

“…even stripped of these supernatural surmisings, there was enough in the earthly make and incontestable character of the monster to strike the imagination with unwonted power. For, it was not so much his uncommon bulk that so much distinguished him from other sperm whales, but, as was elsewhere thrown out- a peculiar snow-white wrinkled forehead, and a high, pyramidical white hump. These were his prominent features; the tokens whereby, even in the limitless, uncharted seas, he revealed his identity, at a long distance, to those who knew him.

The rest of his body was so streaked, and spotted, and marbled with the same shrouded hue, that, in the end, he had gained his distinctive appellation…”–Herman Melville, Moby Dick

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I remember in the biology classes I took in college the way the history of biological scales was laid out to me. The size of the world seemed pretty clear. On the one side lived multicellular creatures like you or I (I presume…and if I’m incorrect, please let me know!), and on the other were the single celled creatures. Single-celled creatures had been hidden from sight for much of human history. Only when van Leeuwenhoek finally brought his lens to their world did we gain the power to see them, for the protozoa were denizens of the microcosmos and could be counted on, for all their multitude of forms, to be microscopic.

Or were they? Anyone who has read my posts here knows I’m obsessed with the way we think of scale in biology. Humans tend to think of ourselves as more or less normal sized creatures from which anything else could be measured as larger or smaller, but this is, after all, not strictly true. To my cat, we are behemoths (who are inexplicably bad at running, hunting, smelling, hearing, or using our claws; but that’s a different post), and to the fruit flies I raised, our apartment is an entire world. On the other end of the spectrum, the rotifers we look at are all multicellular creatures, but they are so small themselves that they are sometimes dwarfed by the ciliates they hunt. And then, there is the Stentor coeruleus, a monster in its taxonomical kingdom.

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The first time I encountered a stentor, I misidentified it as a rotifer. Laksiyer corrected me and called it the king of the ciliates. That wasn’t an S. coeruleus but a smaller variety clinging to a plant; it had the decency to remain in its scale, invisible to my eyes. When I found a specimen of the larger species, it appeared to my naked eye as a dark blue dot hanging on the meniscus of a sample of pond water. When I drew it into my eyedropper, I assumed I was getting an interesting glob of algae or a colony of bacteria. When it moved, I was overjoyed.

Not a white whale, but a blue one, tinted a bright cyan from its partnership with blue-green algae that live in its body. As it moves, it periodically sheds these in bursts of pigmented goop.

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What makes the stentor so interesting to me is not merely that they are a very large single-celled creature. It is what the size of the creature allows us in terms of observing and documenting the microcosmos. Under even a low-powered lens, the organelles of the stentor are clearly visible. In particular, its macronuclei, strung along the length of its body like costume jewelry pearls.

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Even more interesting, you can see the texture of its cellular membrane. Long striations of fibers up and down its body that allow it to change form and stretch to great lengths. You can see the way its mouth curls into a spiral, a divot where the vortex of its cilia leads its prey.

In this way, the stentor also tickles my other obsession on this site: the aesthetics of scientific viewing. The world presented through a light microscope so often seems to be a two dimensional one, but here is a creature that is aggressively three dimensional. Once again, the light microscope becomes a tool for aesthetic and empathetic sight, in addition to, and as a part of, scientific observation. In looking at it, we get a glimpse of what the world looks like at a microscopic scale. We get a view of what the protists living in its world experience when they encounter this great whale.

7 Comments Add yours

  1. Saad says:

    Matt – What a beautiful post. I read it as soon as I woke up in the morning and its such a great way to get re-insprired with stentor – the way you describe it and the reference to moby dick.

    Wonderful!
    Also the videos are just beautiful – you really captured the cyan color quite well and you can see the macronucleus and the fibre network along the body quite well.
    Perhaps you may have already known this – but stentors are known to habituate- which means that they count and learn – that adds another twist to what a single cell can do – without having nerve cells or a brain.

    Cheers,
    Saad

    1. Matthew Rossi says:

      Whoa! They do what?! How do they learn without a brain? I’m still wrapping my head around the notion that things without nervous systems or muscles seem to feel the slide cover and flinch away from it. But they also learn?! That’s incredible.

  2. prabha says:

    Wow, the world of microscopy!!

  3. Manu Prakash says:

    What a wonderful world view @Matt. The blue whale is always stuck in my head now.

    The group of prof Wallace Marshall at UCSF has been reviving stentor as a new animal model system to study the developmental biology of cell; with exciting experiments on habituation (that Saad described). I just wanted to make that pointer; in case you wanted to read more. The work on habituation mechanisms is unpublished (I think).

    Cheers
    Manu

  4. laksiyer says:

    What a wonderful post Matt. I was lost in my recollections of Melville and enjoying every bit of the great “blue whale”. What magnificent views of the cilia, buccal cavity and vacuoles. Truly a giant. The last one seemed like a gigantic vacuole too. Could you grow it ?

    I have been trying to culture ciliates o flate. Been trying to hit upon something that is generic. Hay infusion is really nice but no telling what else grows and whether the ciliate of choice grows. Been trying salt solutions. This Stentor would be perfect as you can see it divide without putting it in the microscope. Given its cyanobacterial symbiont, I suspect a salt solution under lights might be good enough to enrich this. Fantastic post, made my day.

  5. Cristina says:

    Superb! Thanks for sharing such beautiful work!

  6. MananSuri says:

    This is such an amazing post! 🙂

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