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  1. Manu Prakash says:

    Fluorescence microscopy can be implemented in several ways. Can you specifically describe what you are trying to image – based on what you are trying to image; implementation ideas will differ. Can you describe what excitation wavelength you would need.

    See for basic summary of fluorescence microscopy here: https://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html


  2. jstandeven says:

    We are attempting to visualize a Red Fluorescent Protein expression in E.coli. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm. We need a cost effetive(obviously) way to excite as well as choice for filters.

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