DBT Foldscope scheme “Foldscope for diagnosis of rumen acidosis and parasitic infections in cattle”
Here’s a beautiful and detailed picture of the processed Posterior Spiracle of a Chrysomya larvae which was collected from an infected wound of a cow.
The steps involved are:
- The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water.
- It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%).
- The larvae was then kept in a clearing agent ( carbolic acid).
- The cleared larvae was then mounted in DPX mountant and examined under foldscope.
The posterior spiracle of Chrysomya larvae (Figure 1) has a pair of more or less circular spiracles with 3 longitudinal slits (b) , incomplete circumference (a) and no button (c).
Figure 1: Posterior spiracle of Chrysomya species