Cell viability test using Trypan Blue with help of Foldscope

Procedure:

  1. HeLa Cell Harvested by trypsinization.
  2. Resuspended in 1X PBS.
  3. Add equal volume of Trypan Blue(5mg/ml).

    Viable cell under conventional microscope . c - Dead cell
    A -Dead cell under conventional microscope (200X).
    B – Viable cell under conventional microscope (200X) .
    c – Dead cell under Foldscope(140X  with 2X Optical Zoom).
    D – Viable cell under Foldscope(140X  with 2X Optical Zoom).
  4. Observed under Foldscope and conventional microscope.

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