Determination of leaf pigments by using UV-Visible Spectroscopy and leaf structures of Medicinal Plants by using Folds-cope: An Innovative Research Tools.

Mona Saini*, Nutan Rani and Kalawati Saini
Miranda House, University of Delhi,Delhi-110007, INDIA
Corresponding author. Tel: (+91) 1127666983; Fax: (+91) 1127667437;
E-mail: kalawati.saini@mirandahouse.ac.in

Leaf pigment content provides a valuable information about the physiological performance of leaves. We have determined pigments in the leaves of Ficus religiosa (Peepal), Azadirachta Indica (Neem) and Ocimum tenuiflorum (Tulsi) by using UV-Visible spectroscopy and leaf structures of two medicinal plants (Tinospora cordifolia, Origanum majorana) with folds-cope. The images have been taken by connecting the Folds-cope with mobile (Vivo-Y69).  The images of transverse-section (T.S.) of different part of leaves obtained through Fold-scope are comparable with Scanning-Electron Micrograph (SEM). The leaves pigments have been extracted in isopropyl alcohol. On the basis of spectral absorbance (A) at ʎmax  , three pigments ( Chlorophyll- ʎmax-660-680, Carotenoid- ʎmax400-500, , Anthocynine ʎmax550) have been determined with help of Lambert-Beer’s law. Our results show that the concentration of different pigments in leaves vary with sites of location and region of plants. The concentration of all three pigments in the Jaipur region plants have been found very less than the Delhi region. The least amount of all pigments has been found in Azadirachta Indica (Neem) out of all three plants. The amount of pigments in the leaves of Delhi region plants has been found three times of chlorophyll, five time of carotenoid and two times of anthocynine more than the Jaipur  region leaves of same plants. It means uptake of CO2 from environment in photosynthesis by different plants located in different region becomes different. The global warming can be decreased by planting more Ficus religiosa (Peepal) on the earth.

Keywords: Leaf pigment, medicinal plant, Fold-scope, SEM, Concentration.

Leave a Reply