#Indiafoldscopephase1#Fluid circulation in the nymph of Kudzu beetle

First instar nymph of kudzu beetle was observed under foldscope and the outcome was quite good. Pumping and circulation can be clearly seen in this video.

5 Comments Add yours

  1. Manu Prakash says:

    Dear @Santana,

    That’s a beautiful observation. Great work.

    Can you share how you found the nymph and how you prepared the slides..

    Cheers
    Manu

    1. Santana Saikia says:

      Thank you soo much sir.
      Actually I am rearing Kudzu beetle for my project. One first instar nymph was simply put on a clean plain glass slide with the help of a 0 size soft brush and cellotap was used to cover the nymph in such a manner that it forms a bubble,so that the nymph can easily take breath and circulation and pumping can be easily seen. As their body color is very light so circulation is easily can be seen under foldscope

    2. Santana Saikia says:

      And Sir, I placed the nymph dorsally on the slide so that the ventral side face the lens directly .

  2. Manu Prakash says:

    Fantastic. Thanks for sharing.. you can use the edit button on the posts to actually add the sample preparation info on the post. This way readers will know about the wonderful tricks.

    I assume you used the ultra transparent tape cover slip in the kit. If not, give it a try.. we made it to ensure imaging through tape did not cause too much degradation of the image quality.. although glass cover slips are always the best – but more tricky with a live sample.

    Great work!

    Cheers
    Manu

    1. Santana Saikia says:

      Sir, thank u soo much fr the infornation. Will surely try to upld the preparation info.
      Here I have used normal cellotape,but I will try that ultra transparent tape in my next experiment.
      Thank you once again Sir.

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