A suggestion : To use dyes that rotate the plane of polarised light

It’s been quite a long while since I last posted here…

A small play with polarizing filters and the fern rhizome slides provided in deluxe foldscope kits, I came up with these images ::

I’d want to know which dyes were used in staining as these seem to rotate plane of polarised light.

It’d be quite amazing to use such dyes and observe the prepared slides using polarizing filters !

More images would be coming up soon!

3 Comments Add yours

  1. Puchina says:

    Beautiful images!

    I wonder if it’s not so much the dye, but the intrinsic structure of what’s being looked at….carbohydrate polymers in very regular pattern…

  2. laksiyer says:

    Fantastic, @Manu or @JimCybulski might have the answer to this. Why dont you also post the same image without polarization and we will be able to make out if it is the dye or not. Cell wall components might rotate pp light, but such strong signals of only one type of region suggests a dye.

  3. Manu Prakash says:

    Stunning and beautiful. The dye is clearly differentiating cell type – which was one of the big ideas Paul Ehrlich got his nobel prize for in 1908.

    https://www.nobelprize.org/prizes/medicine/1908/ehrlich/biographical/

    I will check to see f the effect is coming from the cell wall or the dye. This is a common slide every Foldscope user gets.

    Cheers
    Manu

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