Dressing up protozoans (Vital Staining): Tetrahymena Vorax Part 2

Have you ever tried to stain ciliates naively, i.e. without knowing any protocol, just adding methylene blue or some such dye? More than often all one sees are shriveled cells that don’t resemble anything like the live ones. For long, microscopists have used vital stains to stain ciliates live and I have always been wanting…