Fun with Fungi

In North America fungi are a mess, taxanomically speaking. Many fungi that look similar to ones found in Europe inherited the same scientific names and this is partly because beyond the fruiting body (mushroom), fungi are pretty hard to get a good look at. A fair number of attributes that are used to discriminate between species need microscopy. DNA sequencing of a conserved region such as the ITS is becoming the gold standard, but its a much harder attribute to access for most amateur mycologist. Access to microscopes for looking at the asci and basidia (sexual reproductive structures) in addition to spore size, shape, and texture could greatly improve observation data on fungi and can help in the identification of new species and/or getting the taxonomy straightened out on mis-labeled species.

I went for a walk around Lake Merritt in Oakland, California to see if any fungi had sprouted up since the past rain and was happy to find one fresh fruiting of Paneolina foenisecii that I was able to use the foldscope to look at its spores:

Paneolina foenisecii and spores (12-14 x 6-7.5 µm from literature)
Paneolina foenisecii and spores (12-14 x 6-7.5 µm from literature)

I had some dried mushroom samples from recent hikes, so I re-hydrated them and also looked to see how well Foldscope did at viewing their spores and asci. I specifically looked at the Ascomycota because the spores tend to be 10µm or greater which seems to be ideal for the Foldscope’s low magnification lens. I have yet to master getting good images with the high magnification lens.

Scutellinia sp.
Scutellinia sp.

 

Helvella vespertina
Helvella vespertina
Peziza proteana ssp. sparassoides
Peziza proteana ssp. sparassoides

Notes on technique: I used a glass slide with a coverslip over a drop of water with the spores/scraped sporing surface. I found that being able to wipe the lens of the Foldscope with some lens paper greatly increased my image quality, but this is probably because I had been getting water with all kinds of stuff in it when I was messing around focusing. I had a desk lamp that I would move the Foldscope in front of until I found the appropriate contrast to get a good image with an iPhone 5. I would zoom in with the cell phone’s camera, push down on the screen to auto-focus lock  and then use the onscreen slider to adjust the exposure. Shooting spores was a little difficult because I tend to hold the Foldscope up vertically and the spores start to slide down inside the coverslip and as I focus I occasionally put pressure on the coverslip causing flushes of water to whisk spores away from my field of view.

Overall I’m really happy with Foldscope’s ability to help see these structures in the field as it could help with in field identifications and decisions about what specimens are worth taking out of the field for further study.

 

5 Comments Add yours

  1. Manu Prakash says:

    Wow wow wow. What a fantastic post. Absolutely gorgeous images. This can occupy all my morning walks now..

    Also; the latest version of Foldscope 2.0 has a focus locking feature – please send me a ping/address and I can send you one to test out and provide more feedback.

    Please share posts often – your work will provide so much motivation to thousands of other Foldscope users.

    I bet a lot of mycologists would be thrilled.

    Cheers
    Manu

  2. laksiyer says:

    Wow. This is fascinating. Please gives us more pointers on what else we need to look at in the fungi. Are the spores themselves used for classification? Is there something we could do with the mycelia? Any staining techniques?

    Also if you have any expertise in slime molds it would be fun to know.
    Cant wait to hear more on your explorations.

  3. Cristina says:

    These photographs are incredibly beautiful! Congratulacions!

  4. damontighe says:

    @Laksiyer –
    Mycologist often use the Q value of spores which is just the ratio of length to width. Typically you’d want to calculate Q values on 30 or more spores, the more the better, statistically speaking. The Q value can be useful in telling species in the same genus apart that might have spores with about the same sort of shape, texture and coloration.

    I reached out to handful of online mycology groups to see if anyone had some techniques they’d like to see on the Foldscope and I can go after those once a list starts coming together.

    So much fun!

  5. laksiyer says:

    Oh thats wonderful. This should be easy to do in Image J. Would be an interesting experiment to first try out on two related species. Will keep that in mind. I must also do that for pollen to see if there are any interesting patterns. Thank you.

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