Observation of onion skin under Foldscope using safranin
| Thu Jun 15 50919 11:40:00 GMT+0000 (Coordinated Universal Time)
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Onion skin under Foldscope -
1. Keep the section in 30% alcohol for 5 minutes. 2. Stain the sections in 1% solution of safranin in 50% alcohol for 30 minutes 3. Dehydrated in 70%, 80% and 90% alcohol, keeping 5 minutes in each. 4. Counterstain 1% light green in 95% alcohol, for 15 seconds. 5. Dehydrate in absolute alcohol for 5 minutes. 6. Pass through alcohol xylil (1:1) mixture keeping the section in it for 5 minutes. 7. Clear in xylol for 10 minutes. 8. Mount it Canada balsam and dry. 9. Place this side on the Foldscope and view it. -
Onion skin staining procedure: •Pour some distilled water into a watch glass. •Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf. •Put the epidermis in the watch glass containing distilled water. •Take a few drops of safranin solution in a dropper and transfer this into another watch glass. •Using a brush, transfer the peel into the watch glass containing the safranin solution. •Let this remain in the Safranin solution for 30 seconds, so that the peel is stained. •Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water. •Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide. •Using the brush, place the peel onto the slide containing glycerine. •Take a cover slip and place it gently on the peel with the aid of a needle. •Remove the extra glycerine using a piece of blotting paper. •Place this glass side on the Foldscope and view it.
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