Methodology:

• Root tips 1 to 2 cm was cut and incubated in Carnoy’s fixative for 15 to 20 minutes.
• Then washed thoroughly in water 2 to 3 times.
• The root tips was transfered in 1N Hydrochloric acid and incubate at 60°C for 5 minutes.
• Stained with acetocarmine for 10 minutes. Transferred onto a clean slide and covered with a cover slip.
• Gently tap the root and observe under the Microscope and Foldscope

Stage Acronyms

Interphase: S

Prophase: 1

Metaphase: 2

Anaphase: 3

Telophase: 4