Mouse Spleen Sections – Bio60_2019
Feb 24, 2019 • 12:34 AM UTC
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cejordan
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The mouse spleen sections appeared slightly fuzzy and monochromatic under the foldscope. The magnitude on the foldscope might not have been strong enough to view the spleen in more detail. The rip in the upper right corner most likely occurred when I was positioning the section on the slide. Firstly, the credit of cutting these mouse spleen sections belongs to Nolan, a research assistant from the lab I work in, who kindly donated these sections for my assignment. The sections were originally submerged in liquid in a well plate to be used for immunofluorescence staining. Using a paint brush, I lifted each section out from my designated well and placed them into water. I then carefully positioned eight of the sections onto a microscope slide with a paintbrush. I had previous experience with painting spinal cord sections; however, this process was more challenging because I used a surgical microscope to watch my movements with more detail. I had never used a microscope when painting slides before and accidentally ripped some of the sections, one of which can be seen in the image.
The slide is meant to be viewed under a powerful fluorescence microscope to view the fluorescent markers on the cells. Under the foldscope, the sections appear monochromatic and look like a consistent texture rather than individual cells. Nevertheless, I enjoyed the challenge of mounting this slide.
The slide is meant to be viewed under a powerful fluorescence microscope to view the fluorescent markers on the cells. Under the foldscope, the sections appear monochromatic and look like a consistent texture rather than individual cells. Nevertheless, I enjoyed the challenge of mounting this slide.
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