Firstly, the credit of cutting these mouse spleen sections belongs to Nolan, a research assistant from the lab I work in, who kindly donated these sections for my assignment. The sections were originally submerged in liquid in a well plate to be used for immunofluorescence staining. Using a paint brush, I lifted each section out from my designated well and placed them into water. I then carefully positioned eight of the sections onto a microscope slide with a paintbrush. I had previous experience with painting spinal cord sections; however, this process was more challenging because I used a surgical microscope to watch my movements with more detail. I had never used a microscope when painting slides before and accidentally ripped some of the sections, one of which can be seen in the image.
The slide is meant to be viewed under a powerful fluorescence microscope to view the fluorescent markers on the cells. Under the foldscope, the sections appear monochromatic and look like a consistent texture rather than individual cells. Nevertheless, I enjoyed the challenge of mounting this slide.