CORNEAL SCRAPING
May 19, 2019 • 11:42 PM UTC
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New comment on your post “CORNEAL SCRAPING IVE VIDEO”
Author: Manu Prakash (IP address: 205.154.255.130, 205.154.255.130)
Email: manup@stanford.edu
URL: http://www.stanford.edu/~manup
Comment:
Dear @Erolab,
Thanks for sharing the video. Can corneal scrapings be done in the
field? What classes of eye infections are often detected this way?
A few tips on slide making for foldscope. in the last step – the slide
(cover slip) should be facing downwards so the lens is looking through
the cover slip. Also, for all my wet samples; I always attach two
pieces of tape covering the edges of the cover slip. This way, when I
insert a slide – it does not slip sideways.
Would love to talk more and understand better the needs in the field.
You can write to me directly at manup@stanford.edu
Wonderful project.
Cheers
Manu
Dear Sir
Thank you very much for
your valuable comments on our corneal scraping scrutinization video. As a
microbiologist working in this diagnostic field for the past 20 years, I must
thank you for your wonderful innovative idea – invention of foldscope and we
are indeed proved of it.
In your mail you have
inquuired whether corneal scraping shall be examined in under the foldscope
field?
Yes, foldscope could be more useful to eye
doctors. I served at Aravind Eye Hospitals Madurai as well as at Coimbatore,
Tamil Nadu, India for more than 5 years. As a diagnostic microbiologist, we
came across some difficulties during corneal sample transport and its
microbiological examination with respect to availability of facilities; trained
staff members (or) microbiologist so as to diagnose the corneal infections.
More importantly, we had to refer corneal infected patients/ cases to other
referral hospitals owing to the lack of cost- effective diagnostic facilities.
In particular, corneal infections have been a serious
ophalmic issue in our country. According
to the World Health Organization, corneal diseases are among the major causes
of vision loss and blindness in the world today, after cataract and glaucoma.( 2 ) In India, it is
estimated that there are approximately 6.8 million people who have vision less
than 6/60 in at least one eye due to corneal diseases; of these, about a
million have bilateral involvement.( 3 , 4 ) It is expected that
the number of individuals with unilateral corneal blindness in India will
increase to 10.6 million by 2020.( 4 ) According to the
National Programme for Control of Blindness (NPCB) estimates, there are
currently 120,000 corneal blind persons in the country. According to this
estimate there is an addition of 25,000-30,000 corneal blindness cases every
year in the country.( 5 ) The burden of corneal
disease in our country is reflected by the fact that 90% of the global cases of
ocular trauma and corneal ulceration leading to corneal blindness occur in
developing countries.( 6 ) Indian
J Community Med . 2013 Oct-Dec; 38(4): 198–206.
Against the indicated facts which we have highlighted in the DBT
project proposal,, I hope your contribution/ invention – Foldscope would
certainly assist to identify and solve some important problems in this
field. Remarkably, for the past 8
months, we have been using foldscope for the rapid examination and
identification microbial pathogens in corneal samples collected from suspected
cases. More importantly, we also generated appropriate data of comparative
studies between normal binocular microscope and the foldscope.
In your mail you have been asked about placing of cover slip on the
slide.
Sir,
we use 10% KOH wet mount for processing , when you use exact amount (volume)
the cover slip will not move; for safer side, we can use tape as you stated.
Sometimes we also use tape for the same.
Fungal
ulcer can be immediately identified using the foldscope, also more useful for
Doctors to treat the patient on the spot.
For
bacterial ulcer, we find some difficulties, because of the size of the bacteria
and ability of the foldscope magnification. Foldscope observation of fungal
filaments from culture using Lacto phenol cotton blue staining (LP) has been
found to be effective.
Here,
we have conducted foldscope hands- on sessions for Erode ophthalmic association
Doctors and trained them to use foldscope. We need to concentrate all the
districts especially rural and urban hospitals.
My
sincere thanks to you for your hard work behind Foldscope invention, we have
been truly sincere in bringing this project to the most essential and important
diagnostic fields so as to help the society. I am very optimistic that with
some improvement and modifications of the current model of the novel device, we
can achieve more in future.
Thanking
you
Author: Manu Prakash (IP address: 205.154.255.130, 205.154.255.130)
Email: manup@stanford.edu
URL: http://www.stanford.edu/~manup
Comment:
Dear @Erolab,
Thanks for sharing the video. Can corneal scrapings be done in the
field? What classes of eye infections are often detected this way?
A few tips on slide making for foldscope. in the last step – the slide
(cover slip) should be facing downwards so the lens is looking through
the cover slip. Also, for all my wet samples; I always attach two
pieces of tape covering the edges of the cover slip. This way, when I
insert a slide – it does not slip sideways.
Would love to talk more and understand better the needs in the field.
You can write to me directly at manup@stanford.edu
Wonderful project.
Cheers
Manu
Dear Sir
Thank you very much for
your valuable comments on our corneal scraping scrutinization video. As a
microbiologist working in this diagnostic field for the past 20 years, I must
thank you for your wonderful innovative idea – invention of foldscope and we
are indeed proved of it.
In your mail you have
inquuired whether corneal scraping shall be examined in under the foldscope
field?
Yes, foldscope could be more useful to eye
doctors. I served at Aravind Eye Hospitals Madurai as well as at Coimbatore,
Tamil Nadu, India for more than 5 years. As a diagnostic microbiologist, we
came across some difficulties during corneal sample transport and its
microbiological examination with respect to availability of facilities; trained
staff members (or) microbiologist so as to diagnose the corneal infections.
More importantly, we had to refer corneal infected patients/ cases to other
referral hospitals owing to the lack of cost- effective diagnostic facilities.
In particular, corneal infections have been a serious
ophalmic issue in our country. According
to the World Health Organization, corneal diseases are among the major causes
of vision loss and blindness in the world today, after cataract and glaucoma.( 2 ) In India, it is
estimated that there are approximately 6.8 million people who have vision less
than 6/60 in at least one eye due to corneal diseases; of these, about a
million have bilateral involvement.( 3 , 4 ) It is expected that
the number of individuals with unilateral corneal blindness in India will
increase to 10.6 million by 2020.( 4 ) According to the
National Programme for Control of Blindness (NPCB) estimates, there are
currently 120,000 corneal blind persons in the country. According to this
estimate there is an addition of 25,000-30,000 corneal blindness cases every
year in the country.( 5 ) The burden of corneal
disease in our country is reflected by the fact that 90% of the global cases of
ocular trauma and corneal ulceration leading to corneal blindness occur in
developing countries.( 6 ) Indian
J Community Med . 2013 Oct-Dec; 38(4): 198–206.
Against the indicated facts which we have highlighted in the DBT
project proposal,, I hope your contribution/ invention – Foldscope would
certainly assist to identify and solve some important problems in this
field. Remarkably, for the past 8
months, we have been using foldscope for the rapid examination and
identification microbial pathogens in corneal samples collected from suspected
cases. More importantly, we also generated appropriate data of comparative
studies between normal binocular microscope and the foldscope.
In your mail you have been asked about placing of cover slip on the
slide.
Sir,
we use 10% KOH wet mount for processing , when you use exact amount (volume)
the cover slip will not move; for safer side, we can use tape as you stated.
Sometimes we also use tape for the same.
Fungal
ulcer can be immediately identified using the foldscope, also more useful for
Doctors to treat the patient on the spot.
For
bacterial ulcer, we find some difficulties, because of the size of the bacteria
and ability of the foldscope magnification. Foldscope observation of fungal
filaments from culture using Lacto phenol cotton blue staining (LP) has been
found to be effective.
Here,
we have conducted foldscope hands- on sessions for Erode ophthalmic association
Doctors and trained them to use foldscope. We need to concentrate all the
districts especially rural and urban hospitals.
My
sincere thanks to you for your hard work behind Foldscope invention, we have
been truly sincere in bringing this project to the most essential and important
diagnostic fields so as to help the society. I am very optimistic that with
some improvement and modifications of the current model of the novel device, we
can achieve more in future.
Thanking
you
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