During my workshop at GGDSD COLLEGE chandigarh I took cottage cheese and suspended it in  water and centrifuged it at 2000 rpm for 2 mins.After 2 mins , I prepared a smear from the supernatant and observed it under foldscope .


 First I stained the smear with methylene blue and observed cocci . They may be the normal microflora of the cheese like Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Streptococcus.  I also found some large sized microbe that I suspected to be yeast. To confirm it, I stained my sample with lactophenol cotton blue.

<figure aria-describedby="caption-attachment-41537" class="wp-caption alignnone" id="attachment_41537" style="width: 225px">
 <img alt="IMG-20180617-WA0011" class="size-medium wp-image-41537 gcloudimg" data-attachment-id="41537" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="IMG-20180617-WA0011" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_41534/IMG-20180617-WA0011.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-41537">
 View under foldscope
 </figcaption>
</figure>

 <img alt="IMG_20180608_122523" class="alignnone size-medium wp-image-41538 gcloudimg" data-attachment-id="41538" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1528460723","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0007446016381236","title":"","orientation":"0"}' data-image-title="IMG_20180608_122523" data-recalc-dims="1" height="169" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_41534/IMG_20180608_122523.jpg" width="300"/>
 yeast

<figure aria-describedby="caption-attachment-41539" class="wp-caption alignnone" id="attachment_41539" style="width: 300px">
 <img alt="IMG_20180608_122414" class="size-medium wp-image-41539 gcloudimg" data-attachment-id="41539" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1528460654","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.00072358900144718","title":"","orientation":"0"}' data-image-title="IMG_20180608_122414" data-recalc-dims="1" height="169" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_41534/IMG_20180608_122414.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-41539">
 Suspected yeast in cheese
 </figcaption>
</figure>


<figure aria-describedby="caption-attachment-41535" class="wp-caption alignnone" id="attachment_41535" style="width: 225px">
 <img alt="IMG-20180617-WA0012" class="size-medium wp-image-41535 gcloudimg" data-attachment-id="41535" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="IMG-20180617-WA0012" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_41534/IMG-20180617-WA0012.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-41535">
 Cocci obtained on staining with methylene blue
 </figcaption>
</figure>

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 This post is open to read and review on The Winnower.
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Cottage cheese

Cottage cheese

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