ISOLATION OF LITTER DECOMPOSING MICROORGANISMS IN MANNAR HILL RANGE – THE WESTERN GHATS
Jul 09, 2018 • 12:20 PM UTC
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RAMYA
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I am E.K. Ramya, working as a Project Assistant under the foldscope project of DBT. Our PI is Dr. S. Sharmila, Assistant Professor, Vellalar College for Women, Erode, Tamil nadu.
Litter decomposition is a process of decaying the dead organic material – with the help of microorganisms. It is one of the crucial role in the forest ecosystem. For decomposition Bacteria and Fungi play a major role. Identification of litter decomposing microorganisms in study area was the 4 th step of my study. For this study, I have collected the litter samples in litter bags during May 2018 and the collected samples are used for further microbial analysis. Analysis of microbes from litter (May 2018):
Litter sample contains mass of microorganisms. It was isolated in the culture medium using Nutrient Agar Medium.
For isolation I have followed 4 important steps:
Step 1: Bacterial Colony formation
Step 2: Isolation of bacteria in Pure culture
Step 3: Gram staining
Step 4: Biochemical test
Step 1: Bacterial Colony formation:
Bacteria grow on solid media as colonies. A colony is known as visible mass of microorganisms originating from single mother cell.
Litter decomposition is a process of decaying the dead organic material – with the help of microorganisms. It is one of the crucial role in the forest ecosystem. For decomposition Bacteria and Fungi play a major role. Identification of litter decomposing microorganisms in study area was the 4 th step of my study. For this study, I have collected the litter samples in litter bags during May 2018 and the collected samples are used for further microbial analysis. Analysis of microbes from litter (May 2018):
Litter sample contains mass of microorganisms. It was isolated in the culture medium using Nutrient Agar Medium.
For isolation I have followed 4 important steps:
Step 1: Bacterial Colony formation
Step 2: Isolation of bacteria in Pure culture
Step 3: Gram staining
Step 4: Biochemical test
Step 1: Bacterial Colony formation:
Bacteria grow on solid media as colonies. A colony is known as visible mass of microorganisms originating from single mother cell.
This pic… shows the Nutrient Agar Media with Beautiful Bacterial Colonies The plate showing the bacterial colonies. I have captured this picture in my microbial laboratory. The pic… also indicates numerous different colonies. The Colonies are occurring in different size & shape. I have calculated number of colonies in single plate with the help of colony counter. Step 2: Isolation of bacteria in Pure culture:
Different bacterial colonies were isolated and inoculated into other separate media. After 4 or 5 sub culturing I have maintained two pure cultures.
Different bacterial colonies were isolated and inoculated into other separate media. After 4 or 5 sub culturing I have maintained two pure cultures.
This pic… shows the unknown bacteria in Nutrient Agar Media – Quadrant Streak plating For separation of gram positive and gram negative I have done Staining process. With the support of foldscope under high magnification I clearly viewed beautiful colour differentiation in gram staining process. Two pure cultures:
Gram positive Gram negative
Gram positive Gram negative
Foldscope view of Rod shaped Gram-positive bacteria– (violet)
Foldscope view of Rod shaped Gram-negative bacteria – (Pink) Biochemical test:
This is the important step for differentiate species from genera.
Biochemical Test for Gram- Positive bacteria
Indole test Methyl Red test Vioges Proskauer test Citrate utilization test Indole test (-ve)
This is the important step for differentiate species from genera.
Biochemical Test for Gram- Positive bacteria
Indole test Methyl Red test Vioges Proskauer test Citrate utilization test Indole test (-ve)
a) No pink ring formation Methyl Red test (-ve)
b) No colour changes (pink) Vioges Proskauer test (+ve)
c) Pink ring formation Citrate utilization test (+ve)
d) Colour changes (Blue) Biochemical Test for Gram- Negative bacteria Indoletest (-ve)
e) No pink ring formation Methyl Red test (-ve)
f) No colour changes (pink) Vioges Proskauer test(-ve)
g) No pink ring formation Citrate utilization test (+ve)
h) Colour changes (Dark blue)
As per the schedule of work plan, half of the work in process. Later I will upload remaining part of the study (Isolation of micro – organisms) with the help of foldscope magnification.
As per the schedule of work plan, half of the work in process. Later I will upload remaining part of the study (Isolation of micro – organisms) with the help of foldscope magnification.
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