Here’s a beautiful and detailed picture of the processed Posterior Spiracle of the Musca domestica Larvae, which was collected from an infected wound.

1. The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water.
2. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%).
3. The larvae was then kept in a clearing agent ( carbolic acid).
4. The cleared larvae was mounted in DPX mountant and  examined under foldscope.

The posterior end of the larvae consists of

• a Pair of brown ‘D’ shaped spiracles
• A chitinised ring (button) and
• 3 sinuous ‘m’ shaped stigmatic slits on each spiracle.