Posterior Spiracle of Maggots ( Musca domestica Larvae)
Aug 29, 2018 • 3:10 AM UTC
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lakshmisundaram
I am Dr.S.Meignanalakshmi, working as Professor, at the Directorate of Centre for Animal Health Studies, TANUVAS, Chennai-51. Working on Foldscope project on "Foldscope for diagnosis of Rumen Acidosis and parasitic infections in cattle" sanctioned by DBT
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Here’s a beautiful and detailed picture of the processed Posterior Spiracle of the Musca domestica Larvae, which was collected from an infected wound.
The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%). The larvae was then kept in a clearing agent ( carbolic acid). The cleared larvae was mounted in DPX mountant and examined under foldscope. The posterior end of the larvae consists of
a Pair of brown ‘D’ shaped spiracles A chitinised ring (button) and 3 sinuous ‘m’ shaped stigmatic slits on each spiracle.
The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%). The larvae was then kept in a clearing agent ( carbolic acid). The cleared larvae was mounted in DPX mountant and examined under foldscope. The posterior end of the larvae consists of
a Pair of brown ‘D’ shaped spiracles A chitinised ring (button) and 3 sinuous ‘m’ shaped stigmatic slits on each spiracle.
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