Posterior Spircale of Chrysomia species

Nov 27, 2018 • 3:13 AM UTC

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Microorganisms

lakshmisundaram

I am Dr.S.Meignanalakshmi, working as Professor, at the Directorate of Centre for Animal Health Studies, TANUVAS, Chennai-51. Working on Foldscope project on "Foldscope for diagnosis of Rumen Acidosis and parasitic infections in cattle" sanctioned by DBT

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DBT Foldscope scheme “Foldscope for diagnosis of rumen acidosis and parasitic infections in cattle”
Here’s a beautiful and detailed picture of the processed Posterior Spiracle of a Chrysomya larvae which was collected from an infected wound of a cow.
The steps involved are:
The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%). The larvae was then kept in a clearing agent ( carbolic acid). The cleared larvae was then mounted in DPX mountant and examined under foldscope. The posterior spiracle of Chrysomya larvae (Figure 1) has a pair of more or less circular spiracles with 3 longitudinal slits (b) , incomplete circumference (a) and no button (c).