Posterior Spircale of Stomoxys species
Oct 04, 2018 • 10:15 PM UTC
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lakshmisundaram
I am Dr.S.Meignanalakshmi, working as Professor, at the Directorate of Centre for Animal Health Studies, TANUVAS, Chennai-51. Working on Foldscope project on "Foldscope for diagnosis of Rumen Acidosis and parasitic infections in cattle" sanctioned by DBT
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Stomoxys commonly known as stable fly resembles the house fly (Musca domestica). Stable flies are obligate blood feeders and primarily attack cattle and horses for a blood meal (Entnemdept.ufl.edu, 2018)
The Stomoxys larvae was collected from an infected wound and was processed.
The steps involved are:
The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%). The larvae was then kept in a clearing agent ( carbolic acid). The cleared larvae was then mounted in DPX mountant and examined under foldscope. The posterior spiracle of Stomoxys has 3 ‘s’ shaped yellow slits (Figure 1) and is lightly sclerotized.
The Stomoxys larvae was collected from an infected wound and was processed.
The steps involved are:
The larvae was boiled in 10% NaOH for about 5 mins and was thoroughly rinsed in water. It was then dehydrated for 2 – 5 mins in increasing concentrations of alcohol ( 70%, 90% and 100%). The larvae was then kept in a clearing agent ( carbolic acid). The cleared larvae was then mounted in DPX mountant and examined under foldscope. The posterior spiracle of Stomoxys has 3 ‘s’ shaped yellow slits (Figure 1) and is lightly sclerotized.
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