Determination of leaf pigments by using UV-Visible Spectroscopy and leaf structures of Medicinal Plants by using Folds-cope: An Innovative Research Tools.
Oct 11, 2018 • 10:51 AM UTC
Unknown Location
140x Magnification
Microorganisms
kksaini
Learn about the author...
92posts
4comments
1locations
Mona Saini*, Nutan Rani and Kalawati Saini
Miranda House, University of Delhi,Delhi-110007, INDIA
Corresponding author. Tel: (+91) 1127666983; Fax: (+91) 1127667437;
E-mail: kalawati.saini@mirandahouse.ac.in Leaf pigment content provides a valuable information about the physiological performance of leaves. We have determined pigments in the leaves of Ficus religiosa (Peepal), Azadirachta Indica (Neem) and Ocimum tenuiflorum (Tulsi) by using UV-Visible spectroscopy and leaf structures of two medicinal plants ( Tinospora cordifolia, Origanum majorana ) with folds-cope. The images have been taken by connecting the Folds-cope with mobile (Vivo-Y69). The images of transverse-section (T.S.) of different part of leaves obtained through Fold-scope are comparable with Scanning-Electron Micrograph (SEM). The leaves pigments have been extracted in isopropyl alcohol. On the basis of spectral absorbance (A) at ʎ max , three pigments ( Chlorophyll- ʎ max-660-680 , Carotenoid- ʎ max400-500, , Anthocynine ʎ max550 ) have been determined with help of Lambert-Beer’s law. Our results show that the concentration of different pigments in leaves vary with sites of location and region of plants. The concentration of all three pigments in the Jaipur region plants have been found very less than the Delhi region. The least amount of all pigments has been found in Azadirachta Indica (Neem) out of all three plants. The amount of pigments in the leaves of Delhi region plants has been found three times of chlorophyll, five time of carotenoid and two times of anthocynine more than the Jaipur region leaves of same plants. It means uptake of CO 2 from environment in photosynthesis by different plants located in different region becomes different. The global warming can be decreased by planting more Ficus religiosa (Peepal) on the earth.
Keywords: Leaf pigment, medicinal plant, Fold-scope, SEM, Concentration.
This post is open to read and review on The Winnower.
Miranda House, University of Delhi,Delhi-110007, INDIA
Corresponding author. Tel: (+91) 1127666983; Fax: (+91) 1127667437;
E-mail: kalawati.saini@mirandahouse.ac.in Leaf pigment content provides a valuable information about the physiological performance of leaves. We have determined pigments in the leaves of Ficus religiosa (Peepal), Azadirachta Indica (Neem) and Ocimum tenuiflorum (Tulsi) by using UV-Visible spectroscopy and leaf structures of two medicinal plants ( Tinospora cordifolia, Origanum majorana ) with folds-cope. The images have been taken by connecting the Folds-cope with mobile (Vivo-Y69). The images of transverse-section (T.S.) of different part of leaves obtained through Fold-scope are comparable with Scanning-Electron Micrograph (SEM). The leaves pigments have been extracted in isopropyl alcohol. On the basis of spectral absorbance (A) at ʎ max , three pigments ( Chlorophyll- ʎ max-660-680 , Carotenoid- ʎ max400-500, , Anthocynine ʎ max550 ) have been determined with help of Lambert-Beer’s law. Our results show that the concentration of different pigments in leaves vary with sites of location and region of plants. The concentration of all three pigments in the Jaipur region plants have been found very less than the Delhi region. The least amount of all pigments has been found in Azadirachta Indica (Neem) out of all three plants. The amount of pigments in the leaves of Delhi region plants has been found three times of chlorophyll, five time of carotenoid and two times of anthocynine more than the Jaipur region leaves of same plants. It means uptake of CO 2 from environment in photosynthesis by different plants located in different region becomes different. The global warming can be decreased by planting more Ficus religiosa (Peepal) on the earth.
Keywords: Leaf pigment, medicinal plant, Fold-scope, SEM, Concentration.
This post is open to read and review on The Winnower.
Sign in to commentNobody has commented yet... Share your thoughts with the author and start the discussion!
0 Applause
0 Comments
