Onion cells stained with Carbol Rose Bengal
Jan 05, 2015 • 11:57 AM UTC
Unknown Location
140x Magnification
Unknown
laksiyer
Human observer of life. https://sukshmadarshin.wordpress.com
94posts
1234comments
5locations
My childhood favorite wet mount is the onion “skin”. The late Walter Dioni had many nice articles on it and how to prepare a good sample. For example, see
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artnov03/wdonion.html
The preparation was done rather carelessly but I was too excited to try out my Foldscope. The epidermis preparation was stained with a drop of Carbol rose bengal (I bought this from the internet) for about 30-45 seconds and then washed with 70% ethanol (CVS) and tap water. Carbol Rose Bengal stains the nucleus brightly and gives a dainty contrast. If you look carefully, you can also see nucleoli. Taking pictures with the Foldscope was a bit rough at first as I was worried I might damage the microscope with the slide, but it is quite hardy, and with a few trials, it got easier. One thing I did was to tape the coverslip onto the slide so that it doesnt slip around while loading.
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artnov03/wdonion.html
The preparation was done rather carelessly but I was too excited to try out my Foldscope. The epidermis preparation was stained with a drop of Carbol rose bengal (I bought this from the internet) for about 30-45 seconds and then washed with 70% ethanol (CVS) and tap water. Carbol Rose Bengal stains the nucleus brightly and gives a dainty contrast. If you look carefully, you can also see nucleoli. Taking pictures with the Foldscope was a bit rough at first as I was worried I might damage the microscope with the slide, but it is quite hardy, and with a few trials, it got easier. One thing I did was to tape the coverslip onto the slide so that it doesnt slip around while loading.
Sign in to commentNobody has commented yet... Share your thoughts with the author and start the discussion!
More Posts from laksiyer
Halteria, the virovore
0 Applause
0 Comments
III. Understanding the earliest farmers: Secondary endosymbiosis, Matryoshka dolls
0 Applause0 Comments
II. Understanding the earliest farmers: The vast diversity of algae.
0 Applause0 Comments
Microbial conflict in action: The fascinating world of amoebae
0 Applause0 Comments
My Dictyostelium story
0 Applause0 Comments
I. Understanding the earliest farmers: The “crops”
0 Applause0 Comments
A busy pond
0 Applause0 Comments
The all-engulfing Sun: Experiments with the heliozoan Actinosphaerium
0 Applause0 Comments
Culturing your favorite ciliate: Paramecium busaria
0 Applause0 Comments
Coleps: The Piraña of the ciliates
0 Applause0 Comments
Rheinberg illumination for Foldscope
0 Applause0 Comments
Ciliate motion: Saltation and its possible reversal in Cyclidium
0 Applause0 Comments
Tetrahymena thermophila mutants: Fat, Mouthless and Balloon
0 Applause0 Comments
Bdelloid rotifer development
0 Applause0 Comments
Games we play: Tetrahymena vs Chlamydomonas. Part 3 of series
0 Applause0 Comments
Dressing up protozoans (Vital Staining): Tetrahymena Vorax Part 2
0 Applause0 Comments
Polymorphism, cannibalism, epigenetics, and biological conflict: The many stories of Tetrahymena vorax – Part I
0 Applause0 Comments
Imaging Tetrahymena thermophila cilia
0 Applause0 Comments
A simple hack for reflection/epi-illumination using the foldscope
0 Applause0 Comments
ASSETT and Foldscope: A marriage made in microcosmos heaven
0 Applause0 Comments