Onion cells stained with Carbol Rose Bengal
Jan 05, 2015 • 11:57 AM UTC
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laksiyer
Human observer of life. https://sukshmadarshin.wordpress.com
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My childhood favorite wet mount is the onion “skin”. The late Walter Dioni had many nice articles on it and how to prepare a good sample. For example, see
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artnov03/wdonion.html
The preparation was done rather carelessly but I was too excited to try out my Foldscope. The epidermis preparation was stained with a drop of Carbol rose bengal (I bought this from the internet) for about 30-45 seconds and then washed with 70% ethanol (CVS) and tap water. Carbol Rose Bengal stains the nucleus brightly and gives a dainty contrast. If you look carefully, you can also see nucleoli. Taking pictures with the Foldscope was a bit rough at first as I was worried I might damage the microscope with the slide, but it is quite hardy, and with a few trials, it got easier. One thing I did was to tape the coverslip onto the slide so that it doesnt slip around while loading.
http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artnov03/wdonion.html
The preparation was done rather carelessly but I was too excited to try out my Foldscope. The epidermis preparation was stained with a drop of Carbol rose bengal (I bought this from the internet) for about 30-45 seconds and then washed with 70% ethanol (CVS) and tap water. Carbol Rose Bengal stains the nucleus brightly and gives a dainty contrast. If you look carefully, you can also see nucleoli. Taking pictures with the Foldscope was a bit rough at first as I was worried I might damage the microscope with the slide, but it is quite hardy, and with a few trials, it got easier. One thing I did was to tape the coverslip onto the slide so that it doesnt slip around while loading.
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