PVC coupon were immersed in garden tank for almost 3 months. Biofilms were formed on the PVC coupon.  The coupon was removed from garden tank and a loopful of the sample was taken and smear was prepared on a glass slide and stained with Gram Stain and observed under Foldscope.
 <img alt="IMG_20181005_155430_HDR" class="alignnone size-medium wp-image-79306 gcloudimg" data-attachment-id="79306" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538754870","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0030769230769231","title":"","orientation":"0"}' data-image-title="IMG_20181005_155430_HDR" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_155430_HDR.jpg" width="225"/>
 <img alt="IMG_20181005_183931_HDR" class="alignnone size-medium wp-image-79307 gcloudimg" data-attachment-id="79307" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538764770","copyright":"","focal_length":"3.57","iso":"116","shutter_speed":"0.0073529411764706","title":"","orientation":"0"}' data-image-title="IMG_20181005_183931_HDR" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_183931_HDR.jpg" width="225"/>
 <img alt="IMG_20181005_184422_HDR" class="alignnone size-medium wp-image-79308 gcloudimg" data-attachment-id="79308" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538765062","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.009009009009009","title":"","orientation":"0"}' data-image-title="IMG_20181005_184422_HDR" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_184422_HDR.jpg" width="300"/>
 <img alt="IMG_20181005_184740_HDR" class="alignnone size-medium wp-image-79309 gcloudimg" data-attachment-id="79309" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538765260","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0071428571428571","title":"","orientation":"0"}' data-image-title="IMG_20181005_184740_HDR" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_184740_HDR.jpg" width="225"/>
 <img alt="IMG_20181005_184901_HDR" class="alignnone size-medium wp-image-79311 gcloudimg" data-attachment-id="79311" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538765341","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.00390625","title":"","orientation":"0"}' data-image-title="IMG_20181005_184901_HDR" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_184901_HDR.jpg" width="300"/>
 <img alt="IMG_20181005_185650_HDR" class="alignnone size-medium wp-image-79313 gcloudimg" data-attachment-id="79313" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538765810","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0058479532163743","title":"","orientation":"0"}' data-image-title="IMG_20181005_185650_HDR" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_185650_HDR.jpg" width="300"/>
 <img alt="IMG_20181005_190059_HDR" class="alignnone size-medium wp-image-79314 gcloudimg" data-attachment-id="79314" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"MI MAX","caption":"","created_timestamp":"1538766059","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0050761421319797","title":"","orientation":"0"}' data-image-title="IMG_20181005_190059_HDR" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_79304/IMG_20181005_190059_HDR.jpg" width="300"/>


 Dr. Poonam Singh/ Srinivasa Sundara Rajan R
 
 CSIR-CECRI
 
 Karikudi

Unstained and stained images of Biofilm from PVC Coupon

Poonam Singh

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