Auramine-O

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Name: Ramya Nambiar
College: CMR National PU college, ITPL campus, Bangalore
Item observed: Auramine O
Description:-
Auramine-O is a diarylmethane dye used as a fluorescent stain. In its pure form it is very soluble in water and in ethanol.
When viewed under the foldscope, it appears as yellow needle crystals. It is a hydrochloride and a fluorescent stain. It along with carbol generates brilliant yellow fluorochrome of bacilli.
It is used for the staining of acid-fast organisms, coccidia. It is also effective in detecting positive cases of tuberculosis. Viewing this under the foldscope gave me a magnified image of every single particle that made it and also gave me a better understanding of this chemical dye.

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