Biofilm Analysis in Drinking Water Distribution System
Nov 19, 2018 • 10:42 PM UTC
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poonamsingh2
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C omparison of Biofilm on PVC coupons in Drinking water distribution system using Foldscope and SEM.
PVC coupons were immersed in drinking water distribution systems for almost 30 days. Analysis of water and biological parameters were done at regular interval of time. The coupons were removed and observed for biofilm formation. The biofilm formation was observed on PVC coupons, which was scraped and transferred to a glass slide and stained with Gram stain and observed under Foldscope. The same coupon was also analysed under SEM. Both the foldscope and SEM results are shown in the figure given below.
Characterization of biofilm was also studied using Fourier-transform infrared spectroscopy (FTIR). FTIR results showed the band at 1255 cm -1 is associated with phosphate of DNA (Whelan et al, 2013). The presence of bacterial proteins within all CaCO 3 crystalline is indicated by strong amide II bind was observed at 1526 cm -1 . The bands in the region 1630-1655 cm -1 were assigned to OH bending of water molecules. The FTIR also showed the presence of biofilm on PVC coupon.
PVC coupons were immersed in drinking water distribution systems for almost 30 days. Analysis of water and biological parameters were done at regular interval of time. The coupons were removed and observed for biofilm formation. The biofilm formation was observed on PVC coupons, which was scraped and transferred to a glass slide and stained with Gram stain and observed under Foldscope. The same coupon was also analysed under SEM. Both the foldscope and SEM results are shown in the figure given below.
Characterization of biofilm was also studied using Fourier-transform infrared spectroscopy (FTIR). FTIR results showed the band at 1255 cm -1 is associated with phosphate of DNA (Whelan et al, 2013). The presence of bacterial proteins within all CaCO 3 crystalline is indicated by strong amide II bind was observed at 1526 cm -1 . The bands in the region 1630-1655 cm -1 were assigned to OH bending of water molecules. The FTIR also showed the presence of biofilm on PVC coupon.
Fourier-transform infrared spectroscopy (FTIR) Results
Dr. Poonam Singh/ Srinivasa Sundara Rajan R
CSIR-CECRI
Karaikudi
Dr. Poonam Singh/ Srinivasa Sundara Rajan R
CSIR-CECRI
Karaikudi
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