Histological studies of somatic embryogenesis in Bacopa monnieri

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KV Saritha

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Somatic embryogenesis has been achieved from leaf explants derived callus culture in Bacopa monnieri on MS medium supplemented with various concentrations of 2,4-D alone. Histological observations of leaf callus showed different stages of embryo development observed under Foldscope.
(A) Embryogenic calli developed from leaf explant of Bacopa monnieri
(B) Pro-embryo development
(C) Globular stage embryo
(D) Heart shape embryo
(E) Torpedo shape embryo

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