Holotrich Protozoa identification in a field setting
Jun 06, 2019 • 1:59 AM UTC
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lakshmisundaram
I am Dr.S.Meignanalakshmi, working as Professor, at the Directorate of Centre for Animal Health Studies, TANUVAS, Chennai-51. Working on Foldscope project on "Foldscope for diagnosis of Rumen Acidosis and parasitic infections in cattle" sanctioned by DBT
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DBT Foldscope scheme – “ Foldscope for diagnosis of Rumen acidosis and Parasitic infection in cattle”.
This post is in response to Manu Prakash’s comment on our previous post (Holotrich protozoa – from Rumen fluid). Rumen fluid was collected from an healthy cow (Figure 1) in a sterile container (figure 2). A drop of rumen fluid was taken and 5 to 10 times the volume of MFS (methyl green Formalin Saline) stain was added ( figure 3). After an incubation time of about 30 mins, the mixture was taken and viewed under Foldscope (Figure 4).
This post is in response to Manu Prakash’s comment on our previous post (Holotrich protozoa – from Rumen fluid). Rumen fluid was collected from an healthy cow (Figure 1) in a sterile container (figure 2). A drop of rumen fluid was taken and 5 to 10 times the volume of MFS (methyl green Formalin Saline) stain was added ( figure 3). After an incubation time of about 30 mins, the mixture was taken and viewed under Foldscope (Figure 4).
Figure 1 – Collection of Rumen fluid
Figure 2: Rumen fluid collected in a sterile container
Figure 3: MFS staining in field setting
Figure 4: Slide viewed under Foldscope
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