The Power of Concentration

My favorite Foldscope experiment is simple: collect pond or lake water and find out what is inside. It is perhaps THE classic microscopy experiment since the days of Leeuwenhoek. Discovering the strange life inside of a single drop of water is always satisfying.

I recently visited Lake Anza in Berkeley’s Tilden Park to collect water and characterize the micro-biodiversity of the place. A drop of lake water under the Foldscope allows you to see much – algae, ciliates, strange types of debris..but you also tend to see the same, most common forms over and over again.

To find a wider diversity of life, I decided to try concentrating my samples. I used a nylon mesh with a 5 micron pore size, folded in into a funnel, and passed maybe 100 mL of lake water through. The 5 micron filter size should allow bacteria to pass through, but should trap many larger single-celled eukaryotes and small metazoa. When the sample had almost all the way passed through the filter, so there was only a few drops remaining in the cone, I collected one of these drops for the Foldscope.

nylon-funnel
Nylon mesh (5 micron pore size) folded into funnel

Within my concentrated sample, I saw an abundance of interesting life forms…

1)First was this cell with rapidly beating cilia all around it:

2) Next, I found a pair of interesting creatures interacting with one another: an orange-ish diatom and a green algae. I was fascinated observing them and condensed all my footage down into the following clips, which show the pair moving together, as well as each species moving individually. They are not irreversibly anchored, as the point of attachment varies, and as you will see towards the end, they eventually separate temporarily, re-connect, and then separate entirely. I do not know if this interaction is rare or not, and am unsure of its purpose.

3) I saw this cluster of algae, with two different shades of green, a darker one and a paler one. Is it two different species or variety within the same species?

4) Finally, I was fascinated by this rapidly flailing creature. At first I thought it was the same ciliate as 1), but then realized that the motions were not 360 degrees around, but were localized to one side. Also, the appendages appear thicker than cilia, making me wonder if this was some kind of larval form.

As always, your thoughts, observations, and identifications are welcomed!

Until next time,

Max

2 Comments Add yours

  1. laksiyer says:

    Great Great videos Max. First one is an Actinosphaerium (also Heliozooan)
    3) Likely a blue green alga (bacterium) like Nostoc (left one in chains) and the other is a eukaryotic alga.
    4). Most fascinating, not figured it out yet.

    Max could you send me a link to buying such a mesh.. are they cheap?

  2. Manu Prakash says:

    Wow Max; I am just so thrilled. A few comments.

    1) Could you please share with the community; which mesh you used. I know for the next foldscope kit; we are thinking of including this mesh. But just some possible vendor from where anyone can acquire a little mesh – would be wonderful. Do you have some pictures of how concentrated your pool of stuff got after the filter.

    2) On @Laks comment; I also thought initially that video(1) is a Actinosphaerium; but I have never seen the filaments oscillate like “cilia”.. so I am still not sure. But again; something is beating at high frequency; but it’s not moving. Big puzzle..

    3) The interaction is absolutely beautiful. The diatom can glide; I am not sure about the algae; could be that the flagela is stuck somewhere. Absolutely remarkable.

    4) Wow; the last is definitely a mystery. Again; cilia are beating but its not going somewhere; which is really puzzling. It’s ciliate for sure; many of them have fused cilia. Thus they could be “membranelles” – which are fused and found around mouth parts. See this for small description.
    https://en.wikipedia.org/wiki/Membranelle

    5) Finally; @Laks and I had come up with a “common nomenclature” for Foldscope data; for unidentified species. Could you please find that post and try to use a similar nomenclature. Might be useful for you in the future.

    You made my day; now I can not sleep; since I will be thinking about these videos.

    cheers
    manu

    cheers
    manu

Leave a Reply