Johnson Retnaraj Samuel Selvan Christyraj

<div class="wp-video" style="width: 720px;">
 <video class="wp-video-shortcode" controls="controls" height="1280" id="video-101225-3" preload="metadata" width="720">
  <source alt="heart-beat.mp4" class="gcloudimg" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101225/heart-beat.mp4" type="video/mp4"/>
  <a alt="heart-beat.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101225/heart-beat.mp4">
   https://microcosmos.foldscope.com/wp-content/uploads/2018/12/heart-beat.mp4
  </a>
 </video>
</div>
<p>
 The earthworm has four pairs of tubular heart, which pump the blood throughout the body through blood vessels. The video shows the clear pumping mechanism of earthworm heart. The documentation was achieved only by the foldscope microscope along with a mobile camera.  (Voice – Ms. G.Mijithra, Junior Research Fellow (DBT foldscope project), Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu)
</p>


Blood flow of Earthworms

<div class="wp-video" style="width: 352px;">
 <video class="wp-video-shortcode" controls="controls" height="640" id="video-101240-2" preload="metadata" width="352">
  <source alt="Blood-flow-in-amputated-worm.mp4" class="gcloudimg" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101240/Blood-flow-in-amputated-worm.mp4" type="video/mp4"/>
  <a alt="Blood-flow-in-amputated-worm.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101240/Blood-flow-in-amputated-worm.mp4">
   https://microcosmos.foldscope.com/wp-content/uploads/2018/12/Blood-flow-in-amputated-worm.mp4
  </a>
 </video>
</div>
<p>
 Earthworm wonderful animal model for Regeneration studies. In this experiment, we interested to study the angiogenesis process during the regeneration of earthworms. We observed the blood flow of earthworms after amputation.
</p>


Blood flow in amputated earthworms

<div class="wp-video" style="width: 1000px;">
 
 <video class="wp-video-shortcode" controls="controls" height="563" id="video-101255-1" preload="metadata" width="1000">
  <source alt="heart-beat-during-amputation.mp4" class="gcloudimg" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101255/heart-beat-during-amputation.mp4" type="video/mp4"/>
  <a alt="heart-beat-during-amputation.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101255/heart-beat-during-amputation.mp4">
   https://microcosmos.foldscope.com/wp-content/uploads/2018/12/heart-beat-during-amputation.mp4
  </a>
 </video>
</div>
<p>
 Earthworms were amputated and observed for the difference in their heartbeat. This video clearly shows the heartbeat of earthworms after the amputation process.
</p>

Earthworm heart beat after amputation

<div class="wp-video" style="width: 720px;">
 
 <video class="wp-video-shortcode" controls="controls" height="1280" id="video-101273-1" preload="metadata" width="720">
  <source alt="bud-formation.mp4" class="gcloudimg" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101273/bud-formation.mp4" type="video/mp4"/>
  <a alt="bud-formation.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101273/bud-formation.mp4">
   https://microcosmos.foldscope.com/wp-content/uploads/2018/12/bud-formation.mp4
  </a>
 </video>
</div>
<p>
 Regeneration of earthworms and blood flow to the regeneration blastema.
</p>
<p>
</p>

Regeneration of earthworms

<div class="wp-block-file">
 <a alt="blood-vessels-of-earthworm.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101826/blood-vessels-of-earthworm.mp4">
  Blood vessels of earthworms
 </a>
 <a alt="blood-vessels-of-earthworm.mp4" class="wp-block-file__button gcloudimg" download="" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_101826/blood-vessels-of-earthworm.mp4">
  Download
 </a>
</div>
<p>
 The Earthworm,
 <em>
  Eudrilus eugeniae
 </em>
 is a segmented worm has many tiny blood vessels, which was observed through foldscope microscope.
</p>


Blood Vessels in Earthworms

<div class="wp-block-file aligncenter">
 <a alt="Amputated-earthworm-under-foldscope.mp4" class="gcloudimg" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_103649/Amputated-earthworm-under-foldscope.mp4">
  Amputated earthworm under foldscope
 </a>
 <a alt="Amputated-earthworm-under-foldscope.mp4" class="wp-block-file__button gcloudimg" download="" href="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_103649/Amputated-earthworm-under-foldscope.mp4">
  Download
 </a>
</div>
<p>
 Amputated earthworm under foldscope microscope and clear view of blood flow to the amputated site.
</p>


Amputated earthworm under foldscope

<figure class="wp-block-image">
 <img alt="Earthworm Intestine" class="wp-image-103666 gcloudimg" data-attachment-id="103666" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Earthworm Intestine" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_103665/Earthworm-Intestine.jpg"/>
 <figcaption>
  Earthworm was observed under foldscope microscope, which clearly shows the earthworm intestine, blood vessel, and setae. Red arrow mark indicates the blood vessel, Yellow arrow mark indicates the presence of setae, and yellow outline shows the earthworm intestine.
 </figcaption>
</figure>


Earthworm Intestine, Blood Vessel and Setea

<p>
 Higher secondary school students from ‘P.A.K. Palanisamy Higher Secondary School, Old Washermenpet, Chennai, Tamil Nadu’ visited our laboratory on 07th Jan, 2019. We demonstrated the assembly and use of foldscope microscope to students. Students excited on seeing the foldscope microscope. Students enjoyed the live documentation of various samples.
</p>


Foldscope Demonstration to School Students

<p>
 Higher secondary school students from ‘Santosh Vidyalaya Matriculation Higher Secondary School, Tambaram East, , Chennai, Tamil Nadu’ visited our laboratory on 07th Jan, 2019. We demonstrated the assembly and use of foldscope microscope to students. Students excited on seeing the foldscope microscope. Students enjoyed the live documentation and digitalization of various samples.
</p>


<p class="has-background has-pale-cyan-blue-background-color">
 On 10th Jan 2019, I went to Sri Sakthi Kailask Women’s College, Salem to deliver a guest lecture. I took few foldscopes along with me during my visit to the college. I demonstrated  the foldscopes, students excited on seeing the slides using foldscopes. Documentation of the specimens by foldscopes through mobile phones attracted the students towards science.
</p>


Demonstration of Foldscopes to College students

<p>
 Centre for Nanoscience and Nanotechnology of Sathyabama Institute of Science and technology has organized Science awareness program on ‘Foldscope Microscope assembly and usage for the School students’ funded by Department of Biotechnology, Government of India on 17-07-2018 at Govt. Hr. Sec. School, Thuraipakkam, Chennai.
</p>
<p>
 To popularize the foldscope microscope to school students, the science awareness program was organized for the Thuraipakkam Govt. Hr.Sec. School, Chennai. School students are excited and enjoyed the flavor of science by seeing the biological samples (Micro-organisms from hands, Onion peal cells, Blood cells, Heart muscle cells, Root tip cells, Cross section of leaf, Earthworm tissues and blood vessels) with the foldscope microscope.
</p>
<p>
 In addition, students understood the seriousness of washing their hands as they have experienced practically that their hands contain tons of micro-organism if hands are not washed properly. Apart from the students, class teachers and the headmaster of the school, delighted on seeing the cheap, paper foldscope microscope as the quality of the image captured by the mobile camera is more clear and fantastic when compared with conventional microscopes.
</p>
<p>
 <img alt="Foldscope image" class="size-medium wp-image-93311 gcloudimg" data-attachment-id="93311" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Foldscope image" data-recalc-dims="1" height="193" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/Foldscope-image.jpeg" style="font-weight: bold;font-size: 1.8rem" width="300"/>
</p>
<p>
 <span style="font-size: 14px">
  Excitement of Students while using foldscopes
 </span>
</p>
<p>
 <img alt="foldscope image 5" class="alignnone size-medium wp-image-93310 gcloudimg" data-attachment-id="93310" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="foldscope image 5" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/foldscope-image-5.jpeg" width="278"/>
</p>
<figure aria-describedby="caption-attachment-93309" class="wp-caption alignnone" id="attachment_93309" style="width: 300px">
 <img alt="foldscope image 4" class="size-medium wp-image-93309 gcloudimg" data-attachment-id="93309" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="foldscope image 4" data-recalc-dims="1" height="181" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/foldscope-image-4.jpeg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-93309">
  Workshop in Thuraipakkam Govt School
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-93308" class="wp-caption alignnone" id="attachment_93308" style="width: 300px">
 <img alt="foldscope image 3" class="size-medium wp-image-93308 gcloudimg" data-attachment-id="93308" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="foldscope image 3" data-recalc-dims="1" height="292" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/foldscope-image-3.jpeg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-93308">
  Workshop in Thuraipakkam School
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-93307" class="wp-caption alignnone" id="attachment_93307" style="width: 300px">
 <img alt="foldscope image 2" class="size-medium wp-image-93307 gcloudimg" data-attachment-id="93307" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="foldscope image 2" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/foldscope-image-2.jpeg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-93307">
  Thuraipakkam Govt school
 </figcaption>
</figure>
<p>
 <img alt="20180717_144935" class="alignnone size-medium wp-image-93302 gcloudimg" data-attachment-id="93302" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"SM-G615F","caption":"","created_timestamp":"1531838975","copyright":"","focal_length":"3.71","iso":"250","shutter_speed":"0.03030303030303","title":"","orientation":"1"}' data-image-title="20180717_144935" data-recalc-dims="1" height="232" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/20180717_144935.jpg" width="300"/>
 <img alt="20180717_145333" class="alignnone size-medium wp-image-93303 gcloudimg" data-attachment-id="93303" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"SM-G615F","caption":"","created_timestamp":"1531839213","copyright":"","focal_length":"3.71","iso":"32","shutter_speed":"0.0072992700729927","title":"","orientation":"1"}' data-image-title="20180717_145333" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/20180717_145333.jpg" width="300"/>
 <img alt="20180717_155927" class="alignnone size-medium wp-image-93304 gcloudimg" data-attachment-id="93304" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"SM-G615F","caption":"","created_timestamp":"1531843167","copyright":"","focal_length":"3.71","iso":"160","shutter_speed":"0.02","title":"","orientation":"1"}' data-image-title="20180717_155927" data-recalc-dims="1" height="177" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/20180717_155927.jpg" width="300"/>
 <img alt="20180717_155938" class="alignnone size-medium wp-image-93305 gcloudimg" data-attachment-id="93305" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"SM-G615F","caption":"","created_timestamp":"1531843178","copyright":"","focal_length":"3.71","iso":"160","shutter_speed":"0.02","title":"","orientation":"1"}' data-image-title="20180717_155938" data-recalc-dims="1" height="175" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/20180717_155938.jpg" width="300"/>
 <img alt="20180718_153821" class="alignnone size-medium wp-image-93306 gcloudimg" data-attachment-id="93306" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"SM-G615F","caption":"","created_timestamp":"1531928301","copyright":"","focal_length":"3.71","iso":"100","shutter_speed":"0.02","title":"","orientation":"1"}' data-image-title="20180718_153821" data-recalc-dims="1" height="201" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_93300/20180718_153821.jpg" width="300"/>
</p>


Foldscopes to School childrens &#8211; Thuraipakkam Govt School

<p>
 <img alt="Live tracking the pattern of regeneration in earthworms" class="alignnone size-medium wp-image-99903 gcloudimg" data-attachment-id="99903" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Live tracking the pattern of regeneration in earthworms" data-recalc-dims="1" height="108" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_99895/Live-tracking-of-regeneration-in-earthworms.jpg" width="300"/>
</p>
<p>
 Live tracking the pattern of regeneration and angiogenesis using foldscopes: The earthworm
 <em>
  Eudrilus
 </em>
 eugeniae was amputated before clitellum and blastema was seen along with blood vessel formation was documented at 0th,12th and 24th hrs.
</p>


Live tracking the pattern of regeneration and angiogenesis

<p>
 <img alt="Blood vessel formation in regeneration blastema" class="alignnone size-medium wp-image-99910 aligncenter gcloudimg" data-attachment-id="99910" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Blood vessel formation in regeneration blastema" data-recalc-dims="1" height="214" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_99914/Blood-vessel-formation-in-blastema.jpg" width="300"/>
</p>
<p>
 Blood vessel formation in the regeneration blastema of an amputated earthworm. The red asterisk indicates the amputated site, whereas yellow asterisk indicates the formation blood vessel in the regeneration blastema.
</p>


Blood vessel formation in the regeneration blastema

<p>
</p>
<p>
 <img alt="Blood vessel and blood flow in earthworms" class="size-medium wp-image-99925 aligncenter gcloudimg" data-attachment-id="99925" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Blood vessel and blood flow in earthworms" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_99924/Blood-vessel-and-blood-flow.jpg" width="236"/>
</p>
<p>
 The earthworm,
 <em>
  Eudrilus
 </em>
 eugeniae is a wonderful animal model for regeneration studies.  The blood vessel and blood flow of the earthworms are clearly visible under foldscope microscope. The red arrow indicates the blood vessel and blood flow.
</p>


Johnson Retnaraj Samuel Selvan Christyraj

Earth

Posts

Foldscopes to School childrens – Thuraipakkam Govt School

Live tracking the pattern of regeneration and angiogenesis

Blood vessel formation in the regeneration blastema

Blood vessel and blood flow in earthworms

Blood flow of Earthworms

Blood flow in amputated earthworms

Earthworm heart beat after amputation

Regeneration of earthworms

Blood Vessels in Earthworms

Amputated earthworm under foldscope

Earthworm Intestine, Blood Vessel and Setea

Foldscope Demonstration to School Students

Foldscope Demonstration to School Students

Demonstration of Foldscopes to College students

Foldscopes to School childrens – Thuraipakkam Govt School

Live tracking the pattern of regeneration and angiogenesis

Blood vessel formation in the regeneration blastema

Blood vessel and blood flow in earthworms

Blood flow of Earthworms

Blood flow in amputated earthworms

Earthworm heart beat after amputation

Regeneration of earthworms

Blood Vessels in Earthworms

Amputated earthworm under foldscope

Earthworm Intestine, Blood Vessel and Setea

Foldscope Demonstration to School Students

Foldscope Demonstration to School Students

Demonstration of Foldscopes to College students

Foldscopes to School childrens – Thuraipakkam Govt School

Live tracking the pattern of regeneration and angiogenesis

Blood vessel formation in the regeneration blastema

Blood vessel and blood flow in earthworms

Locations

Statistics

Type of Sample

Foldscope Lens Magnification