Methodology:

• 10ml of human blood sample was collected and mixed well with glass beads for 30 minutes.
• 5 ml of Histopaque was placed in a 15 ml centrifuge tube.
• 1:1 ratio of Blood and PBS was carefully added onto the Histopaque-1077
• Centrifuge the tubes for 30 min at 100 x g in 4 °C in a swing-out bucket.
• Aspirate the buffy coat formed in the interphase between histopaque and medium.
• Wash and centrifuge the cells for 100 x g for 10 min twice with 10 ml of PBS.
• The cells are then stained with Giesma Stain and observed under foldscope and microscope.

PBS- Isotonic Phosphate Buffer Saline Solution