Methodology:
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10ml of human blood sample was collected and mixed well with glass beads for 30 minutes.
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5 ml of Histopaque was placed in a 15 ml centrifuge tube.
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1:1 ratio of Blood and PBS was carefully added onto the Histopaque-1077
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Centrifuge the tubes for 30 min at 100
x g
in 4 °C in a swing-out bucket.
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Aspirate the buffy coat formed in the interphase between histopaque and medium.
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Wash and centrifuge the cells for 100 x g for 10 min twice with 10 ml of PBS.
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The cells are then stained with Giesma Stain and observed under foldscope and microscope.
PBS- Isotonic Phosphate Buffer Saline Solution