<p>
 Methodology:
</p>
<ul>
 <li>
  10ml of human blood sample was collected and mixed well with glass beads for 30 minutes.
 </li>
 <li>
  5 ml of Histopaque was placed in a 15 ml centrifuge tube.
 </li>
 <li>
  1:1 ratio of Blood and PBS was carefully added onto the Histopaque-1077
 </li>
 <li>
  Centrifuge the tubes for 30 min at 100
  <em>
   x g
  </em>
  in 4 °C in a swing-out bucket.
 </li>
 <li>
  Aspirate the buffy coat formed in the interphase between histopaque and medium.
 </li>
 <li>
  Wash and centrifuge the cells for 100 x g for 10 min twice with 10 ml of  PBS.
 </li>
 <li>
  The cells are then stained with Giesma Stain and observed under foldscope and microscope.
 </li>
</ul>
<p>
 PBS- Isotonic Phosphate Buffer Saline Solution
</p>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20181220_145558__01" class="wp-image-107872 gcloudimg" data-attachment-id="107872" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"ONEPLUS A6000","caption":"","created_timestamp":"1545317759","copyright":"","focal_length":"4.25","iso":"125","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="IMG_20181220_145558__01" height="413" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_107847/IMG_20181220_145558__01.jpg" width="418"/>
  <figcaption>
   Mononuclear cells under Microscope
  </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20181220_150958__01" class="wp-image-107873 gcloudimg" data-attachment-id="107873" data-comments-opened="1" data-image-meta='{"aperture":"1.7","credit":"","camera":"ONEPLUS A6000","caption":"","created_timestamp":"1545318599","copyright":"","focal_length":"4.25","iso":"400","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="IMG_20181220_150958__01" height="322" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_107847/IMG_20181220_150958__01.jpg" width="418"/>
  <figcaption>
   <br/>
   Mononuclear cells under Foldscope
  </figcaption>
 </figure>
</div>


Observation of Mononuclear Cells

Anika bms

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