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Observation of Mononuclear Cells

| Fri, Dec 21, 2018, 1:00 AM



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Methodology:

  • 10ml of human blood sample was collected and mixed well with glass beads for 30 minutes.
  • 5 ml of Histopaque was placed in a 15 ml centrifuge tube.
  • 1:1 ratio of Blood and PBS was carefully added onto the Histopaque-1077
  • Centrifuge the tubes for 30 min at 100 x g in 4 °C in a swing-out bucket.
  • Aspirate the buffy coat formed in the interphase between histopaque and medium.
  • Wash and centrifuge the cells for 100 x g for 10 min twice with 10 ml of PBS.
  • The cells are then stained with Giesma Stain and observed under foldscope and microscope.

PBS- Isotonic Phosphate Buffer Saline Solution

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Mononuclear cells under Microscope
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Mononuclear cells under Foldscope


Locations



Categories

Type of Sample
unknown
Foldscope Lens Magnification
140x

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