We followed the following procedures for nematode extraction:

Collect the soil sample about 500g from rhizophere region of the plant. Sample was placed in the tray and remove debris and stones and add water at required amount to make solution and allow it for 10 minutes. Later, the soil solution was pass on to the different mesh size sieves are 60, 100, 150, 200 and 360 and finally pour the solution out. Collect the sediments settled in all the meshes using water and filtered it by using filter/tissue paper which should be placed above the wire mesh. Later the wire mesh containing filter paper was placed as such it should slightly touching on the water in the petriplate and allowed for 12 hours. So, that nematode moves towards water. After 12 hrs, pick the nematode using steromicroscope and prepare slide as usual and observe under Foldscope.