dbt foldscope

Aspergillus niger observed under foldscope

<figure class="wp-block-image">
 <img alt="IMG_20190105_144524" class="wp-image-120366 gcloudimg" data-attachment-id="120366" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Lenovo A7010a48","caption":"","created_timestamp":"1546699525","copyright":"","focal_length":"3.68","iso":"1593","shutter_speed":"0.099997","title":"","orientation":"1"}' data-image-title="IMG_20190105_144524" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120351/IMG_20190105_144524.jpg"/>
 <figcaption>
  Nymph
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="IMG_20190105_145042" class="wp-image-120367 gcloudimg" data-attachment-id="120367" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Lenovo A7010a48","caption":"","created_timestamp":"1546699843","copyright":"","focal_length":"3.68","iso":"1241","shutter_speed":"0.08","title":"","orientation":"1"}' data-image-title="IMG_20190105_145042" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120351/IMG_20190105_145042.jpg"/>
 <figcaption>
  Head region
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="IMG_20190105_145249" class="wp-image-120368 gcloudimg" data-attachment-id="120368" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Lenovo A7010a48","caption":"","created_timestamp":"1546699970","copyright":"","focal_length":"3.68","iso":"1299","shutter_speed":"0.060003","title":"","orientation":"1"}' data-image-title="IMG_20190105_145249" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120351/IMG_20190105_145249.jpg"/>
 <figcaption>
  <br/>
  Dark brown in color with numerous spines covering the body
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="IMG_20190105_145409" class="wp-image-120369 gcloudimg" data-attachment-id="120369" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Lenovo A7010a48","caption":"","created_timestamp":"1546700050","copyright":"","focal_length":"3.68","iso":"1201","shutter_speed":"0.050005","title":"","orientation":"1"}' data-image-title="IMG_20190105_145409" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120351/IMG_20190105_145409.jpg"/>
</figure>


Citrus blackfly  (Aleurocanthus woglumi) viewed under foldscope

<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20190326_143536" class="wp-image-138638 gcloudimg" data-attachment-id="138638" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi Note 5 Pro","caption":"","created_timestamp":"1553271170","copyright":"","focal_length":"3.81","iso":"250","shutter_speed":"0.0064516129032258","title":"","orientation":"1"}' data-image-title="IMG_20190326_143536" height="410" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_138630/IMG_20190326_143536.jpg" width="496"/>
  <figcaption>
   Soil Sample was collected from Groundnut Field and Followed the Procedure of Cobb’s sieving  and Decanting Method to extract the nematodes.
  </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20190326_143612" class="wp-image-138641 gcloudimg" data-attachment-id="138641" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi Note 5 Pro","caption":"","created_timestamp":"1553271092","copyright":"","focal_length":"3.81","iso":"250","shutter_speed":"0.0076923076923077","title":"","orientation":"1"}' data-image-title="IMG_20190326_143612" height="431" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_138630/IMG_20190326_143612.jpg" width="420"/>
  <figcaption>
   Here,  Plant Parasitic Nematode population are very Low, we only found Saprophytic Nematode which is long and highly mobile compared to parasitic nematode.
  </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20190322_160836 (1)" class="wp-image-138645 gcloudimg" data-attachment-id="138645" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi Note 5 Pro","caption":"","created_timestamp":"1553270916","copyright":"","focal_length":"3.81","iso":"100","shutter_speed":"0.0058479532163743","title":"","orientation":"1"}' data-image-title="IMG_20190322_160836 (1)" data-recalc-dims="1" height="394" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_138630/IMG_20190322_160836-1.jpg" width="203"/>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="IMG_20190322_161024" class="wp-image-138647 gcloudimg" data-attachment-id="138647" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi Note 5 Pro","caption":"","created_timestamp":"1553271024","copyright":"","focal_length":"3.81","iso":"100","shutter_speed":"0.0062111801242236","title":"","orientation":"6"}' data-image-title="IMG_20190322_161024" data-recalc-dims="1" height="238" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_138630/IMG_20190322_161024.jpg" width="508"/>
 </figure>
</div>
<h4>
 We followed the following procedures for nematode extraction:
</h4>
<p>
 Collect the soil sample about 500g from rhizophere region of the plant. Sample was placed in the tray and remove debris and stones and add water at required amount to make solution and allow it for 10 minutes. Later, the soil solution was pass on to the different mesh size sieves are 60, 100, 150, 200 and 360 and finally pour the solution out. Collect the sediments settled in all the meshes using water and filtered it by using filter/tissue paper which should be placed above the wire mesh. Later the wire mesh containing filter paper was placed as such it should slightly touching on the water in the petriplate and allowed for 12 hours. So, that nematode moves towards water. After 12 hrs, pick the nematode using steromicroscope and prepare slide as usual and observe under Foldscope.
</p>


A saprophytic Nematode  extracted from Groundnut Soil Sample was seen under Foldscope at APAC, Kalavai

Rhizopus sporangium with Sporangiospores isolated from decaying bread  sample

Coxa of Embid &#8211; groundnut pest

Hair like rod grown laterally on Striped mealy bug, Host &#8211; Hibiscus

<figure class="wp-block-image">
 <img alt="tuberose leaf spot- curvularia.." class="wp-image-168215 gcloudimg" data-attachment-id="168215" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi S2","caption":"","created_timestamp":"1563186239","copyright":"","focal_length":"3.81","iso":"160","shutter_speed":"0.0083333333333333","title":"","orientation":"0"}' data-image-title="tuberose leaf spot- curvularia.." src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_168197/tuberose-leaf-spot-curvularia...jpg"/>
 <figcaption>
  septate brown hyphae with curved 3-4 celled conidia
 </figcaption>
</figure>


Tuberose leaf spot caused by Curvularia lunata

<figure class="wp-block-image">
 <img alt="rice blast.." class="wp-image-168230 gcloudimg" data-attachment-id="168230" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi S2","caption":"","created_timestamp":"1563884979","copyright":"","focal_length":"3.81","iso":"100","shutter_speed":"0.0076335877862595","title":"","orientation":"0"}' data-image-title="rice blast.." src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_168223/rice-blast...jpg"/>
 <figcaption>
  pyriform shaped conidia with a distinct basal hilum
 </figcaption>
</figure>


Rice blast caused by Magnaporthe grisea observed under foldscope

<figure class="wp-block-image">
 <img alt="red rot&amp;#8230;." class="wp-image-168236 gcloudimg" data-attachment-id="168236" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi S2","caption":"","created_timestamp":"1562837161","copyright":"","focal_length":"3.81","iso":"100","shutter_speed":"0.0027700831024931","title":"","orientation":"0"}' data-image-title="red rot…." src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_168234/red-rot.....jpg"/>
 <figcaption>
  cylindrical shaped conidia of colletotrichum
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="red rot&amp;#8230;." class="gcloudimg"/>
</figure>


Conidia of Colletotrichum gloeosporoides causing Anthracnose disease in mango

<figure class="wp-block-image">
 <img alt="penicillium.." class="wp-image-168246 gcloudimg" data-attachment-id="168246" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"Redmi S2","caption":"","created_timestamp":"1563030321","copyright":"","focal_length":"3.81","iso":"100","shutter_speed":"0.0078740157480315","title":"","orientation":"0"}' data-image-title="penicillium.." src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_168241/penicillium...jpg"/>
 <figcaption>
  hyaline conidiophores with phialides giving brush like appearance
 </figcaption>
</figure>
<p>
</p>


Penicillium isolated from citrus

Egg of fruit fly from bitter gourd

Globose sporangia with collumella of Mucor sp. isolated from decayed bread

Rose pollen grains observed under foldscope

Ant observed under foldscope

Maize Pollen observed under foldscope

<figure aria-describedby="caption-attachment-97224" class="wp-caption alignnone" id="attachment_97224" style="width: 300px">
 <img alt="IMG_20181127_095252" class="size-medium wp-image-97224 gcloudimg" data-attachment-id="97224" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1543312372","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.008695652173913","title":"","orientation":"0"}' data-image-title="IMG_20181127_095252" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_97223/IMG_20181127_095252.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-97224">
  Rhizopus isolated from jackfruit
 </figcaption>
</figure>
<p>
 <img alt="IMG_20181127_095253" class="alignnone size-medium wp-image-97225 gcloudimg" data-attachment-id="97225" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1543312373","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.008695652173913","title":"","orientation":"0"}' data-image-title="IMG_20181127_095253" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_97223/IMG_20181127_095253.jpg" width="300"/>
</p>


dbt foldscope

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Aspergillus niger observed under foldscope

Citrus blackfly (Aleurocanthus woglumi) viewed under foldscope

A saprophytic Nematode extracted from Groundnut Soil Sample was seen under Foldscope at APAC, Kalavai

Rhizopus sporangium with Sporangiospores isolated from decaying bread sample

Locations

Statistics

Type of Sample

Foldscope Lens Magnification