Cell Viability of Animal Cell Line
Sep 18, 2019 • 11:05 PM UTC
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VaniBMSCE
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SKBR-3, a breast cancer cell was prepared on a hemocytometer and observed under Foldscope. Trypan blue staining was done and both viable and dead cells can be seen.
Procedure:
(1) Place 100 µl of cell
suspension in a cryo-vial.
(2) Add equal parts of 0.4%
trypan blue dye to the cell suspension to obtain a 1 to 2 dilution (example: 50
µl of cells to 50 µl of trypan blue) and mix by pipetting up and down.
(3) With the cover slip already
in place, fill one side of a hemacytometer counter with the cell suspension by
placing the tip of the pipette at the notch.
Blue cells are the nonviable
cells.
Procedure:
(1) Place 100 µl of cell
suspension in a cryo-vial.
(2) Add equal parts of 0.4%
trypan blue dye to the cell suspension to obtain a 1 to 2 dilution (example: 50
µl of cells to 50 µl of trypan blue) and mix by pipetting up and down.
(3) With the cover slip already
in place, fill one side of a hemacytometer counter with the cell suspension by
placing the tip of the pipette at the notch.
Blue cells are the nonviable
cells.
Viable cells as seen under Foldscope
Non-viable Animal cells under Foldscope
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