Vanishree Rathod

<p>
 SKBR-3, a breast cancer cell was prepared on a hemocytometer and observed under Foldscope. Trypan blue staining was done and both viable and dead cells can be seen.
</p>
<p>
 Procedure:
</p>
<p>
 (1) Place 100 µl of cell
suspension in a cryo-vial.
</p>
<p>
 (2) Add equal parts of 0.4%
trypan blue dye to the cell suspension to obtain a 1 to 2 dilution (example: 50
µl of cells to 50 µl of trypan blue) and mix by pipetting up and down.
</p>
<p>
 (3) With the cover slip already
in place, fill one side of a hemacytometer counter with the cell suspension by
placing the tip of the pipette at the notch.
</p>
<p style="text-align:center">
 Blue cells are the nonviable
cells.
</p>
<p>
</p>
<div class="wp-block-image">
 <figure class="aligncenter">
  <img alt="Capture1" class="wp-image-171325 gcloudimg" data-attachment-id="171325" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Capture1" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_171324/Capture1-1.png"/>
  <figcaption>
   Viable cells as seen under Foldscope
  </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="Capture2" class="wp-image-171326 gcloudimg" data-attachment-id="171326" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Capture2" data-recalc-dims="1" height="619" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_171324/Capture2-1.png" width="494"/>
  <figcaption>
   Non-viable Animal cells under Foldscope
  </figcaption>
 </figure>
</div>


Cell Viability of Animal Cell Line

<p>
 Thin sections of mouse pancreas were stained by H&amp;E and observed under Foldscope and Microscope.
</p>
<p>
 Beta cells and Islet endothelial cell can be seen with Foldscope.
</p>
<p>
 Beta cells,  Islet endothelial cell , Pancreatic acini and interlobular duct can be seen under compond microscope.
</p>
<p>
 Pancreatic acini is stained darker and has a fibrous wall.
</p>
<div class="wp-block-image">
 <figure class="aligncenter">
  <img alt="Capture3" class="wp-image-171348 gcloudimg" data-attachment-id="171348" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Capture3" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_171331/Capture3.png"/>
  <figcaption>
   Beta cells under Foldscope
  </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
  <img alt="Capture4" class="wp-image-171349 gcloudimg" data-attachment-id="171349" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Capture4" data-recalc-dims="1" height="363" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_171331/Capture4.png" width="479"/>
  <figcaption>
   <br/>
   Islet endothelial cell
   <br/>
   under Foldscope
  </figcaption>
 </figure>
</div>
<figure class="wp-block-image">
 <img alt="Capture5" class="wp-image-171350 gcloudimg" data-attachment-id="171350" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Capture5" data-recalc-dims="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_171331/Capture5.png"/>
 <figcaption>
  Under microscope beta cells ( bold black circle),
  <br/>
  Pancreatic acini (black circle), interlobular duct  ( white circle) and
  <br/>
  Islet endothelial cell ( blue box) can be observed.
 </figcaption>
</figure>


Vanishree Rathod

Earth

Posts

Cell Viability of Animal Cell Line

Mouse Pancreas for Histological Analysis

Locations

Statistics

Type of Sample

Foldscope Lens Magnification