Understanding single lens microscopy using the Foldscope, part I – lens mag and how to measure

Nov 16, 2017 • 6:34 AM UTC

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140x Magnification

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David Walker

Amateur microscopist. Co-founder of the microscopy hobbyist website www.microscopy-uk.org.uk established in 1995 and editor of its monthly e-zine Micscape. Have a particular interest in single lens microscopy and own two commercial Leeuwenhoek replicas (80x and 100x biconvex lenses). Enjoy using them to repeat studies of subjects reported by Van Leeuwenhoek and comparing with images from a modern microscope.

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Part 1 – Lens magnification and how to measure it (Image does not seem to embedding at the native scale and text overlapping image. Original here . As well as exploring the microscopic world with the Foldscope, it also allows studies of the principles of single lens microscopy. The stated magnification of the lens supplied is 140X. For a single lens its magnification is defined as that observed for a subject when viewed at 250 mm from the lens. This is approximately 10 inches and is taken as the closest distance an unaided normal human eye can focus on an object. This is worth trying with two people. View as close as possible (without spectacles if worn) say text on a page while it still remains in focus. Ask a colleague to measure the distance from the eye to the page (take care with rulers near eyes, measure from the side of the head!). The exact distance measured will vary somewhat. This distance is important in microscopy as the image we see using the Foldscope is not a real image, it is called a virtual image. It is this image that the eye sees ca. 250 mm away even though our eyes are actually very close to the lens. We can measure the mag of the lens ourselves using the type of set up below. Use a semi-darkened room. Support the Foldscope vertically e.g. with wood blocks, books or clamp stand at a measured 250 mm from a wall or vertical pale card. Prepare a slide using the 0.5 mm grid supplied with the Foldscope kit. Alternatively, if own a micrometer slide, use this which typically has finer 0.1 divisions. Focus the scale by eye then mount as shown and refocus a little for sharpest image. To project an image the slight refocus changes the image from a virtual to a real image in order to project it. My result. Five x 0.1 mm units on the wall was 71 mm. This is how much the 0.5 mm field has been magnified. So just divide this measured distance Lm by the actual size La. i.e. Lm / La = 71 / 0.5 = 142 X. That is a close agreement to the stated value of 140 X given we have not used very exact measurements. Owners of the beta trial kit with the higher mag 430 X lens can try the same. This is likely to require the finer scales on a micrometer slide of 0.01 mm.
Tip: If the projected scale is too dull to measure, darken the room completely or move the lens closer to the wall at distance D, say 125 mm, where the image will be brighter and Lm will be smaller. The equation below can then be used which corrects for the fact that a measured size on the wall at say 125 mm will be half that measured at 250 mm.
M = (250 x Lm) / (D x La)
David
(Edited Dec. 9th 2017 to correct the equation to use if project at distances smaller than 250 mm. Also corrected equation in the image text.)

Understanding single lens microscopy using the Foldscope, part I – lens mag and how to measure

David Walker

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