Observation of Stained Blood Smear Using Foldscope

Dec 11, 2018 • 12:16 AM UTC

Unknown Location

140x Magnification

Microorganisms

Jitendra Satija

Learn about the author...

8posts

6comments

1locations

The blood smear was stained with Leishman stain and observed under the foldscope thereafter. The Leishman stain consists of two chemical groups, i.e. Azure B (basic dye) and Eosin Y (acidic dye). The erythrocytes stain pink in color due to Eosin Y as hemoglobin is eosinophilic (acidophilic), whereas the Azure B is a cationic dye which gives purple color to the nucleus and blue/light blue to the cytoplasm. This procedure and foldscopic exercise can be utilized to identify and differentiate leucocytes, malaria parasites, and Trypanosoma.