Pollen Hunters project-II : Pollen roster

Update 6/6/2016. Made the table scrollable for computers and phones. I took help from this page.
Update 4/20/2016. Reducing column numbers to fit new format
Update 11/2/2015. Added Contributors column.
Update (10/2/2015): Added plants that were observed in India, in August 2015. I also have an extensive analysis of trichomes of the Indian plants. Please visit here for more details. Largest pollen 120 microns and smallest one about 3 microns. The average is about 41 microns. Here is a histogram of the size distribution.

Update (6/8/2015): Added 17 more plants. Table format revised. Plants grouped based on broad taxonomic relationships. Pollen sizes added for most of the new entries. Need help for identification in more places now.

Update (4/26/2015): About 10 more plants added, names revised. Some are not known to me. If you know of them please help. Also minor change in protocol and table format.

April 12 (first post): Continuing from my earlier post and inspired by several others who have been diligently taking pictures of flowers and pollen in the foldscope microcosmos page, I thought I would collect as many different types of pollen as possible from the domesticated and wild flora around me (North-east USA), so that I could compare them with my pollen traps. At first, all I did was to pick the flower, foldscope the petal and stain the pollen with a dilute solution of safranin O (.05%). However after looking at some 10 flowers, I realized that the staining process was altering the pollen shape (see this post for details). So I have now moved to making a .05% Safranin in Glycerine (purchased from a local pharmacy). The purpose of the glycerine is just hydration for the pollen to take a more full shape, the dye just adds contrast. More precisely, I drop the petal and pollen on a glass slide, and put a transparent tape on it (the paper slides are great too) to get the details of the petal and unstained/untreated pollen, and then I lift up the tape, put a drop of Safranin in glycerine, spread it around and foldscope it again. Glycerine is messy, so here are a few suggestions. Drop the glycerine on the slide and cover it with a coverslip. Wrap it in a paper towel and place a weight of about 3-5 g for about 20 minutes to remove excess glycerine, wipe the edges, and then seal the ends with tape.

After commencing with great gusto, I realized that this would be a never ending endeavor, and sometimes each flower or plant has many other interesting features. More embarrassingly, my botany is weak and so I thought it would be great to collaborate with the community to annotate the plants or even add to the table. You can send me pictures and details  (Your names will also be listed on the table, see comments for sending). Include a picture of the flower, a foldscoped petal and a foldscoped pollen untreated and if possible treated with glycerine (with stain optional). I also plan to add pollen morphology and other features that catch my attention into the notes.

I realized that for this to be comprehensive, the only way out was to be systematic and so I decided to organize my observations as a table using simple html, This can be constantly updated as my own botany improves. I find this easier than combining it as a picture in picasa (which is what I was doing, and have also done here for some pictures). Also, after the first iteration of this process, I now realize that it is important to view untreated pollen to see the extent of hydration or staining. This page will be constantly updated and hopefully with your inputs the protocols, methods and presentation will only improve. Looking forward to your feedback. Lets do this together.

Notes: For pollen classification, I am using the following website.  There is also the comprehensive Pollen EM database. For North American flowers, I am using the connecticut wild flower database.

Click on thumbnail for high-res image. Table is scrollable horizontally. Computer users click on the table and use your arrow keys. Phone users swipe the table to see more columns.

Flower Flower Petal Untreated pollen Hydrated/stained pollen Common/Species name
(Family)
Contributed by Notes
Angiosperms
Monocots
daff1 daff2 daffodil1 daffodil4 Daffodil (Narcissus
(Amaryllidaceae)
)
LI  Most common flower in early  spring. National flower of Wales. In honor of an old favorite poem of Wordsworth. “I wandered lonely as a cloud…” Pollen size ~65 microns.
spiderlillyplant spiderlillypetal spiderlillypollen Spider lilly
(Amaryllidaceae)
LI  Observed in Chennai, India, August 2015. Didnt get very good shots as it was done on the fly. Pollen size ~ 50 microns.
Valparai-whiteplant Valparai-petal Valparai-whitepollen Some kind of spiderlilly
(Amaryllida)
LI Observed at a tea shop in Valparai, India, August 2015. Pollen size 20-25 microns. Nice yellow color.
muscari muscari2 muscari3 muscari4 Muscari; Grape Hyacinth
(Asparagaceae)
 LI Commonly observed in gardens early spring. Flowers in a raceme. Sticky substance in flower.
whitefl1 whitefl2 whitefl3 whitehyacinth4 White Hyacinth
(Asparagaceae)
LI Fleshy white flowers on raceme,  with lots of liquid when cut. Also seems to cause mild irritation when held for long (perhaps oxalic acid). Hydrated pollen show the different steps in the hydration process. Stains are taken up late in the hydration process.
 tuberose       Tuberose, Polianthes tuberosa
(Asparagaceae)
 Mitali Patil For more details see Mitaali’s post
western-blue-eyed-grass-flower westernblueeyedgrasspetal western-blue-eyed-pollen Western Blue-eyed Grass; Sisyrinchium bellum;
(Asparagaceae)
 Joshua Guild For more details see Josh’s post
    Hosta; Arum-like
(Araceae)
Manu Flowers arranged on a fleshy spadix. And pollen stuck together.  For more details, read Manu’s post
Hosta; Funkia
(Asparagaceae)
LI Commonly found as a household ornamental. Blooms late spring (June) in DC area. Note the petals retain a lot of water.
Dicots
dandelion1 april20 dandelion-stack dandelion4 Dandelion; Taraxacum officinale;
(Asteraceae)
LI Blooms Early-Mid-spring. Rapid cycle. Commonly found as weed and on road sides. Only ray florets. Spherical pollen where exine shows spiny structures.
compo1 compo2 compo3  compos3  ?
(Asteraceae)
LI Compositan flower that blooms late spring on roadsides. White disk floret petals. Pollen size ~ 10 microns.
yelloaster yellowasterpetal yellowasterpollen ?
(Asteraceae)
LI Observed in topslip, Tamil Nadu, August 2015. The stamens of the ray florets are full of trichomes. Pollen size ~20 microns
purplecompositanplant purplecompositantrichomes puplecompositanpollen Centratherum punctatum ;
(Asteraceae)
LI (Identified by inaturalist Lek) Observed in Parambikulam tiger reserve, India, August 2015. Purple compositan. Two types of trichomes on petals and one type on stamen. Pollen size ~20 microns. Probable invasive species
purple1 blue2 Untreated pollen blue3 ? (Unknown family) LI  Blooms early spring, dc area. Possibly a cherry. Didnt observe too many details.
papp1 pollen-of-various-flowers1-2 legume1 legume2 Purple/Red deadnettle; Lamium purpureum
Lamiaceae
LI Commonly observeweed and in wild. Blooms early to mid spring. About 6  inches tall. Extensive trichomes of a single type all over. Flowers early spring. Pollen tubes in some of them. Note hydration of pollen with Glycerine for > 30 minutes makes them spherical.
purplestamenflower purplestamenpetal purplestamenpollen Indian  privet  (Clerodendrum inerme)
also (Volkameria inermes) (Lamiaceae formerly Verbenaceae))
LI Common ornamental observed in Chennai, August 2015. Large purple pollen. Size ~40-50 microns. Apparently has medicinal properties
blue1 blue-blue1 vinca3 vinca4 Periwinkle (Vinca minor)
(Apocyanaceae)
LI Creeper, blooms early spring, dc area. Lots of pollen per flower.
arali-1 arali-2 arali-pollen Common oleander/???? (Nerium oleander)
(Apocyanaceae)
LI Observed in Chennai, August 2015.Commonly grown garden plant in chennai and also the source of floral offerings to dieties. Known to be toxic. Stamens have trichomes. Pollen ~30 microns.  The corolla tube had a second set of petals with pink streaks. Incredibly trichomes are seen emerging from the pink part to the white and more so towards the base of the flower. Why this asymmetry?
yellow-arali-flower yellow-arali-petal yellow-arali-pollen Oleander/???? (Nerium oleander)
(Apocyanaceae)
LI Yellow Arali observed in Chennai, August 2015. The yellow Arali has spear-like trichomes at the base of the petal. Large pollen ~ 90 microns
yellow1 yellow2 forsythia1 forsythia2 Forsythia
(Oleaceae)
LI Shrub, flowers early spring, dc area.
parijatam-flower parijatam-petal parijatam-pollen Parijatam/?/coral jasmine (Nyctanthes arbor-tristis)
(Oleaceae)
LI A plant grown all over Chennai and used for making floral offerings to deities (Observed August 2015). It blooms at night. The flower doesnt last long when plucked and fades away rapidly by day. It has white petals and an orange petal stalk. A trichome was observed in the orange part. Pollen ~ 60 microns. Official flower of the State of West Bengal, India.
Jasmina magniflora (Oleaceae) Mitali Patil From Ratnagiri, India. For more details refer to her post
cherry1 cherry2 cherry-blossom1 cherry4 Cherry (Prunus serrulata)
(Rosaceae)
LI Flowers early spring, dc area.  The untreated pollen has a nice yellow color. After staining the pollen swells up and it appears that the outer wall may even rupture. For example, in the stained specimen, there is a small sphere next to the big one, the small one is likely the broken exine of the pollen.
portlaurel1 portlaure2 portlaurel3 Portugal Laurel
(Rosaceae)
LI Blooms mid spring. Flowers on a spike. Note that even though it is related to the cherry, the pollen shape is so different. Flowers Mid-spring
rosid1 rosid2 rosid3 Wild rose? (Rosa)
(Rosaceae)
LI Looks like a wild rose to me with sweet smelling flowers. Blooms in gardens and in parks mid spring. Pollen 30-40 microns in size.
spring-beauty1 spring-beauty2 spring-beauty4 spring-beauty3 Spring beauty(Claytonia virginica)
(Portulacaceae)
LI Observed in Great Falls, MD, early spring. Pollen inaperturate and easily observed in the purple anthers without staining. Pistil very pretty with many projections in the style. Observed pollen on pistil.
white1 white2 Untreated pollen greatfalls3 ?
(?)
LI Observed in Great Falls, MD, early spring. Stained the pollen with a very very dilute safranin but the structure was very clear.
white-cluster1 whitecluster2 Untreated pollen whitecluster3 ?
(?)
LI Observed in Great Falls, early spring. Looks like strawberry, but am not sure.
magnolia1 tepal magnolia2 pollen-staining-hp Magnolia virginiana
(Magnoliaceae)
LI Blooms early Spring. Large flowers with rubbery tepals and a sweet smell.
southernmagnolia1 southernmagnolia2 southernmagnolia3 Southern Magnolia (Magnolia grandiflora)
(Magnoliaceae)
LI Blooms late spring and early summer. Flower has a sweet, lemon-like smell. Very large tepals and thick leaves. Pollen about 80 microns in size.
blue1 blue2 blue3 blue4 Speedwell (Veronica)
(Plantaginaceae)
LI Tiny  flower, less than an inch. Petals with blue streaks. Interesting trichome pattern in petals. Trichomes only present in one half of the petal. Nice blue anthers (see first picture in hydrated pollen).
bakopa1 bakopa3 bakopa2 Bakopa
(Plantaginaceae)
LI Petals have trichomes closer to the base, perhaps glandular. Blooms continuously starting mid-spring.
purple1 purple2 purple3 purple4 Rhododendron
(Ericaceae)
LI  Blooms early spring.
mutant1 mutant2 mutant3 Azalea-? (Rhododendron)
(Ericaceae)
LI There is a homeotic mutation in this flower where 1-3 stamens form a petal above the anthers, as reported in a previous post. Blooms in mid-spring Pollen about 20 microns in size.
whiteinflo1 whiteinflo2 whiteinflo3 whiteinflo4 ?
(?)
LI Pollen with nice surface features.
maple1 maple2 maple3 redmaple3 Red Maple (Acer rubrum)
(Sapindaceae)
LI Blooms early spring. Red pigment easily leaks out.
goldenraintree1 goldenraintree3 goldenraintree2  goldenraintreepollengly Goldenrain tree/Pride of India (Koelreuteria paniculata)
(Sapindaceae)
LI Blooms late spring and starts fruiting early summer. Four petals asymmetrically distributed growing on a panicle. Fruit is a bladder-like pod. Base of some petals have orange spots. Beyond the orange spots at the very base of the petal are long trichomes in a very dense thicket. Pollen size ~ 20 microns
      Curry Flower Murraya koenigii (Sapindaceae) Mitali Patil From Ratnagiri, India. For more details refer to her post
oak1 oak2 Stained pollen Birch (Betula)
(Betulaceae)
LI Blooms early spring.
dogwood1 dogwood2 dogwood3 dogwood4 Flowering dogwood (Cornus florida)
(Cornaceae)
LI Flowers mid-spring. 4 Bracts surrounding tiny flowers.
spots1 spotscollage spots3 spots4 ?
(?)
LI Some kind of mint with really pretty petals. Trichomes in petals at the base nearer to the stamens and pistils.
oxallis1 oxalis2 oxalis3 oxalis4 Common yellow woodsorrel (Oxalis stricta)
(Oxalidaceae)
LI Heart shaped trifoliate leaves, that closed when plucked. They apparently close at night. Also used in cooking imparting a tart taste.
greenflowers1 greenflowers2 greenflowers3 greenflowers4 ?
(?)
LI Green flowers in a cluster. Blooms mid spring
honeysuckle1 honeysuckle3 honeysuckle3.2 Honeysuckle (Lonicera japonica)
(Caprifoliaceae)
LI Mid-spring bloomer that continues into late spring and possibly summer. Nice perfumed flowers. Usually found as a weed.  Fairly large pollen (50 microns) with interesting surface features. Trichomes all over the anthers and at the base of the petal. Trichomes quite long ( ~300 microns)
robinia1 robinia2 robinia3 itchy4 Black locust (Robinia pseudoacacia)
(Fabaceae)
LI Blooms mid-spring. Fairly large trees. Sweet perfumed flowers in a cluster. Typical legume flowers with each petal of distinct size.
whiteclover1 whiteclover2 whiteclover3 White clover (Trifolium repens
(Fabaceae))
LI Commonly found in lawns. Starting blooms late spring, dc area. Flowers in a bunch at the tip of a flower stalk/peduncle. Flower show a typical legume-like asymmetry. Blooms mid-spring. Pollen size ~  30 microns
orange-1 orange-petal orange-pollen Peacock flower (Caesalpinia pulcherrima)
(Fabaceae)
LI Small shrub observed in Chennai, August 2015. Stamens are extra long. Large pollen in tetrads (~50 microns in size), like the Catalpa pollen. Are these closely related or is this convergence? Convergence I think.  Didnt observe any obvious trichomes.
kanni1 kanni-trichome  kannipollen Golden shower tree/Kannikonna (Malayalam) (Cassia fistula)
(Fabaceae)
LI State flower of Kerala observed in Chennai, August 2015. Blooms around Onam. Flowers in a raceme and look like a grape-bunch. Although there are multiple types of anthers, I could get pollen from the tallest and the some of the smaller ones. Pollen size ~ 40 microns. I didnt realize during sampling that some could be sterile.  A single trichome type in petal.
gulmohar1 gulmoharpetal gulmoharpollen Flambuoyant tree (Delonix regia)
(Fabaceae)
LI A kind of Gulmohar tree. Caesalpinid legume observed in Chennai, August 2015. Commonly found across Chennai. One of the leaves shows variegation in the petal color suggestive of gene silencing. Large yellow pollen with rough surface and ~50 microns in size. Trichomes only on pistil (Need a better pic)
butea-composite butea-trichome butea-pollen1 Flame of the forest/????(Butea monosperma)
(Fabaceae)
LI Was in full bloom in the month of August, 2015 in Valparai. Striking tree. Flowers have leathery petals with large pollen (~40 microns). A very interesting trichome shape was observed.  See this post for more details.
mimosa1 mimosa2 mimosa3 Touch me not (Mimosa pudica)
(Fabaceae)
LI 3-5 micron pollen. Smallest pollen I have seen this far (I had to get a shot of two pollens side by side to get a size estimate). The plant was at the edge of the Parambikulam Tiger reserve (August 2015), definitely an invasive species, though not as prevalent. Stamens dominate the flower. Only got an image of the stalk of the stamen. Couldnt really see any trichomes on the fly.
      Ononis natrix (Fabaceae) Mitali Patil From Ratnagiri, India. For more details refer to her post
itchy1 itchy2 itchy3 itchy4 ?
(?)
LI Very itchy plant, probably Rosaceae. Trichomes all over the petals and leaves. Petal trichomes over 500 microns long. Pollen size ~ 40 microns. Weed like distribution. Blooms mid-spring
catalpa1 catalpa2 catalpa3 catalpa3 Catalpa
(Bignoniaceae)
LI Blooms late spring. The tree was over 50 feet tall. Petals have some interesting color transitions. Pollen in some kind of tetrads ~ 50 microns. A little thrip was seen in the flower.
vinetrumpet1 vinetrumpet2 vinetrumpet3 vinetrumpetgly Trumpet vine (Campsis radicans)
(Bignoniaceae)
LI Blooms late spring and continues blooming through summer. Fairly large orange trumpet shaped petal. There was several thrips in it. Lots of liquid/nectar at the base of the trumpet. Petals also had large stomata-like openings at the base, wonder if the nectar is secreted through it. Pollen size ~ 50-60 microns.
yellow1-flower yellow1-trichomes yellow1-flower-homeotic Yellow trumpet bush (Tecoma stans)
(Bignoniaceae)
LI Observed in Chennai, August 2015.Commonly grown as a  shrub. South american import. Trichomes all over petal and along edge. One of the versions had a homeotic mutation where the stamen was convered to a petal. I couldnt get any pollen and so I have inserted the homeotic mutation, where one of the stamens has been transformed into  a petal.
tritrichome1 tabebuia-trichomes tabebuia-pollen Trumpet tree (Tabebuia berteroi)
(Bignoniaceae)
LI Observed in Chennai, August 2015. Most remarkable trichome distribution. The Yellow part of the corollar tube has several trichomes. The purple top has branched trichomes at the edge. Pollen ~ 30 microns in size. Imported species
corktree-flower cork-trichomes  corktree-pollen Indian cork tree (Millingtonia hortensis
(Bignoniaceae))
LI Extremely fragrant flowers observed in Chennai August 2015. Trichomes all over the petal and can be seen quite visibly. They are usually branched. The distinguishing feature of the petals are that there are four of them and one of them is cleaved/clefted. Pollen ~40-50 microns.
fabaceae1 fabaceae2 fabaceae3 ?
(Fabaceae)
LI Blooms late spring. Fabaceae is my best guess based on leaf and flower shape. Pollen size ~20 microns.
nightshade1 nightshade2 nightshade3 Nightshade (Solanum dulcamara)
(Solanaceae)
LI Purple flowers with berries. Petals have two green “eyes” at the base. The cells around the central vein are rectangular. They are distinguished from the even more toxic deadly nightshade by their floral arrangement. Fused stamens with a carpel sticking out from the center. Pollen ~ 10-15 microns. Easily released even with a gentle push.
datura1 datura-trichome2 datura-pollen Datura/Angel’s trumpet  (Datura sp.)
(Solanaceae)
LI Observed in Chennai, August 2015.A toxic/medicinal plant commonly found on the roadside. Petal thick and leathery. Pollen size ~40-50 microns, Trichomes along edge of petal.
shalini1 shalini2 shalini3 shalini3 Japanese Stewartia (Stewartia pseudocamellia)
(Theaceae)
LI Blooms late spring. Plant 6 feet tall and attracts bees. The white petals have a mild fragrance and leathery in texture. Sepals covered entirely in trichomes. Flower neatly falls off with the carpel on the plant which develops into a dehisced grey fruit with five-fold symmetry. Pollen ~50 microns. Thrips associated with the flower in addition to bees.
teaflower1 tea-petal teapollen2 Tea (Camellia sinensis)
(Theaceae)
LI Flower picked from Valparai, India in August 2015. A lone flower. Lots of stamen, nice yellow pollen of size about 40 microns.
teaflower1 tea-petal tea-petal Japanese camellia(Camilla japonica)
(Theaceae)
Honomi For more details refer to @Honomi’s post
scarletflowercollage scarletpetalcollage scarletpollen Scarlet Pimpernel (Anagallis arvensis)
(Primulaceae)
LI Blooms towards the end of spring and continuously over summer. Flowers about a 1/4 inch wide and nice scarlet color. . When the sun is bright and the sky clear the flowers are open. At other times, during a rain or when the sky is somewhat overcast, or early morning and in evenings, the petals are closed.  Also known as Shepherd’s weatherglass. Glandular trichomes along petal edge that is not touching adjacent petals.
neem1 neempetal neempollen Neem (Azadirachta indica)
(Meliaceae)
LI Observed in Chennai, August 2015.Flowers in a panicle. One  of the hairiest petals with two types of trichomes. Pollen 40-50 microns. It struck me that the pollen were so large for such a small flower.
 lantana-flower lantanatriccoll lanatana-pollen Wild sage (Lantana camara)
(Verbenacea)
LI Common invasive species in Valparai. Observed in August 2015. Trichomes of two types were seen. Pollen size 40-50 microns.  See previous post for more details on the invasive species problem
spiderwort-flower spiderwort-petal spiderwortpollen Dew flower/Spiderwort (Trandescantia)
(Commelinaceae)
LI Spiderwort, observed in a closed state at Pollachi, August 2025. Remakrable trichomes that reminds me of a midnight exploration of this plant with Manu at the National geographic building, DC area. Pollen size ~40-50 microns.
purple1flower purple1trichomecollage purple1pollen Sapphire showers (Duranta erecta)
(Verbenaceae)
LI Observed at Pollachi, India in August 2015. Pollen size 30-40 microns. remarkable trichomes at edge of petal. An invasive plant species in India.
hibiscusflower hibiscuspetal hibiscuspollen Stained pollen Hibiscus(Malvaceae) LI Observed in the suburbs of Pennsylvania, July 2015. Giant pollen ~120 microns. The petal cell shapes differ between the light orange and the dark orange parts.
hibiscusplant hibiscuspetal hibiscuspollen Hibiscus(Malvaceae) Paul Joseph Hibiscus from Kochi, India. For more details refer to post
    Althaea Cannabina (Malvaceae) Mitali Patil From Ratnagiri, India. For more details, especially related to interesting trichome patterns refer to her post
crepe-myrtle-flower crepe-myrtle-petal crepe-myrtle-pollen crepe-myrtle5 Crape Myrtle (Lagerstroemia sp.)
(Lythraceae)
LI Blooms last for long from end of summer to end of fall. Petals in many colors. Pollen size ~40 microns
     Vygies (Mesembryanthemum clavatum) (Aizoacea) Lydia-Marie Joubert Spectacular succulents growing in the middle of a desert. For more details refer Lydia’s post.

Pollen on left, trichomes on right
Stained pollen Pig-face/Ice-plant (Carpobrotus)
(Aizoaceae)
Manu Prakash Interesting trichome patterns and physiological adaptations. For more details, refer Manu’s post.
 balsam  

Image of pollen in anthers (Not stained)
Impatiens balsamina (Garden balsam)  (Balsaminaceae) Maithili Sawant Interesting exploding pods. For more details, refer Maithili’s post.
purple2 purple2-trichomes purple2pollen ?
(?)
LI Observed between Coimbatore and Pollachi, August 2015. Pollen size 40-50 microns, quite an interesting shape. At least two types of trichomes. One with a spherical top and the other one straight.
whitewithyellowplant whitewithyellowpetal whitewithyellowpollen ?
(?)
LI White flower with a yellow center observed in Chennai, India, August 2015.  Pollen size 20-25 microns. The edge of the petal had bumps that might be trichomes but am not too sure they are.
Flower succulent-trichome succulent-pollen ?
(?)
LI Flower from a succulent plant observed at R. Bhanumathi’s place, Chennai, in August 2015. Dont have a picture of the plant but they show wonderful pollen and trichomes. The flower may have been 1/2 inch wide while the orange pollen was about 50 microns in size. The succulent petal shows some purple spots. No picture of flower though.
bluepollenflower bluepollentrichome bluepollenpollen ?
(?)
LI Observed in Topslip, India, August 2015. Really large pollen ~60 microns in size. Two types of trichomes, one of which is beautifully branched.
Gymnosperms
Flower Petal image Untreated pollen Hydrated/stained pollen Common/Species name (Family) Contributed by Name/Notes
pine1 pine3 pine4 Spruce (Piceae)
(Pinaceae)
LI 1. Resin released upon plucking of the male cone. Fairly large pollen (50 microns).
spinypine1 spinypine2 spinypine3 (Pinaceae) LI 2. Pollen ~ 50 microns.
Work in progress
Name Petal image Untreated pollen chillipollen-gly Green Chilli pepper (Capsicum sp.)
(Solanaceae)
LI Name/Notes
Name Petal image Untreated pollen Stained pollen Name
(Family)
Contributed by Name/Notes

16 Comments

  1. Manu Prakash says:

    This is fantastic start of the “Pollen Hunters Project”. This is phenomenal – and also so refreshing and empowering to see. I see flowers all around me – and imagine the flowers that we have never seen..

    Can you mention in the comments:
    1) How we should send you data?
    2) If we can add meta information about the plants catalogued – say when people find peculiar notes about these plants.
    3) Could you list your source for Safranin so other users can use the exact same technique.

    I have been thinking of trying a new website – hypothes.is – but since I have never actually used it; we will wait to encourage others to use it.

    Cheers
    Manu

  2. laksiyer says:

    Hi Manu. I thought this would be a great opportunity to involve everyone. and my botany is abysmal.
    Regarding specific:
    1. Data can be sent to me at pollenhunter@yahoo.com (I just created this account– note yahoo, there is someone else with the same gmail name)
    Pictures can be sent as attachments to the account or weblinks.
    2. Metadata about plants can also be sent to the yahoo account.
    3. I got my Safranin O from Home Science Tools (http://www.hometrainingtools.com/safranin-o-stain-15ml/p/CH-SAFRAN/). It is a 1% aqueous solution and I have been using a 1:20 dilution, although I suspect one can easily go to 1:100.
    4. Perhaps there are better stains. I am exploring food dyes suspended in propylene glycol 🙂

    It would be great if there is an open collaborative page.

  3. laksiyer says:

    Hi Manu: I added more details of my protocol into the text. I looked at hypothes.is and it ] looks really interesting. Something like this is what we need with 100s of volunteers. I cant imagine the depth of data when we get floral information from across the world– all foldscoped. I was just reminded of an article I contributed to my college publication many years ago (Amateurs arise), I found it finally on the web. http://wenku.baidu.com/view/7c252e6f1eb91a37f1115c6f.html
    With an innovation like the foldscope, this can become a reality.

    On another note, I just saw this editing and bibliography demo of F1000 Research, where one can write papers collaboratively. It is just fantastic.

  4. laksiyer says:

    Also I am noticing that untreated pollen has a somewhat more dehydrated shape as compared to the stained pollen. The stained pollen swell up considerably in some instances. I need to play around with this process. Perhaps staining with alcohol based solution may be better?

  5. Manu Prakash says:

    @laksiyer: the expansion comment reminds me of recent “expansion microscopy” to improve resolution. Take a look: http://m.sciencemag.org/content/347/6221/543.abstract

    Cheers
    Manu

  6. laksiyer says:

    Hi Manu. That paper is absolutely fascinating.

  7. jonk4m says:

    This is not only extensive, but well thought out. Thank you for the great work to show students in my area!

    1. laksiyer says:

      @Jonk4m. Thank you. As you can see this page is constantly evolving as I myself struggle to understand the best protocol and practices, being only an amateur. I hope you and your students can join in, add to the page pr make one yourself, or perhaps experiment with the methodology.

  8. laksiyer says:

    I have found a very interesting article on how to stain pollen in a way that can be easily done at home. I noticed that Dave Walker of Microscopy UK also recommends a similar process (http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artjul99/pollen.html). In this I need to dissolve an appropriate amount of Safranin in 50:50 glycerine:H2O. Will try it out and update, perhaps in a separate article.
    The article can be directly accessed at
    http://www.pollinationecology.org/index.php?journal=jpe&page=article&op=download&path%5B%5D=187&path%5B%5D=57

  9. Manu Prakash says:

    Incredible. I have no other words to describe it. I must immediately join in your efforts.

    Cheers
    Manu

  10. Lydia-Marie Joubert says:

    This is truly inspiring, and illustrates the way science can be advanced by amateurs. It also captures some of the orderliness of Botanical classification, and the conservative features that are used in taxonomy. Great work!

  11. Dino Ott says:

    Fantastic work – I love this project.
    I was wondering where the beautiful colourful background comes from in your images?
    Is that also due to the staining or do you use a special type of illumination?

    1. laksiyer says:

      Hi Dino. Thanks. I hope you can join in. Regarding the colors, there are three sources
      1. Whenever I image the pollen over the petal (if I get lucky as it saves me making another slide), the color of the petal determines the background. The second source is safranin which contributes to the pink. The third is the LED itself, which basically appears to be a blue LED fluorescing white and depending on the position of the LED, you can get a blue light instead of white. I dont always like this one, but dont control it too much.

  12. Dino says:

    @laksiyer: thanks for your reply on the origin of the colors. I have observed the same regarding the blue/yellow colors from the LED you mentioned. I reckon this happens when the condenser lens produces an image of the light emitting area of the LED.
    Your images are stunning and the colors make them even more pretty – a nice side effect.

  13. Manu Prakash says:

    @Dino/ @Laks – I wonder if the color can be tuned. For my marine samples; I like the blue hue in my background 🙂

    cheers
    manu

  14. laksiyer says:

    @Manu/@Dino If there can be an alignment adjustment after the focus that would be great.

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