DBT Foldscope Phytoplankton Protocol
Oct 11, 2019 • 9:08 AM UTC
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Mahima Jaini
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We developed this protocol as part of
our DBT foldscope project on #Andamanplankton. The methods are based on
commonly used techniques for sampling marine phytoplankton and have helped easily
reveal Noctiluca, Ceratium, and
various dinoflagellate and diatom species from the inshore waters of the
Lohabarrack Sanctuary in South Andaman (Permit No. CWLW/WL/134(D)/272).
Methodology: 1 litre sample of
seawater is taken from surface and preserved by adding 1ml of Lugol’s Iodine
solution. [Lugol’s Iodine Solution: 20 gm KI + 10 gm I 2 + 200 ml
distilled water + 20 ml glacial acetic acid, stored in dark glass container].
The sample is then allowed to settle for atleast 24 hours. After 24 hours the
community composition of the settled plankton can be observed using the
foldscope. Simple slides of the settled biomass can by created using the
provided foldscope paper or film slides.
#IndiaFoldscopePhase1
– DBT Foldscope Category B Project Details:
Project
Title: Integrating microscopy and satellite oceanography to understand the
dynamics of planktonic communities around the Andaman Islands
Principal
Investigator: Mahima Jaini, Marine Research Officer, Dakshin Foundation
Location:
Andaman Nicobar Environment Team, Wandoor, South Andaman, India
our DBT foldscope project on #Andamanplankton. The methods are based on
commonly used techniques for sampling marine phytoplankton and have helped easily
reveal Noctiluca, Ceratium, and
various dinoflagellate and diatom species from the inshore waters of the
Lohabarrack Sanctuary in South Andaman (Permit No. CWLW/WL/134(D)/272).
Methodology: 1 litre sample of
seawater is taken from surface and preserved by adding 1ml of Lugol’s Iodine
solution. [Lugol’s Iodine Solution: 20 gm KI + 10 gm I 2 + 200 ml
distilled water + 20 ml glacial acetic acid, stored in dark glass container].
The sample is then allowed to settle for atleast 24 hours. After 24 hours the
community composition of the settled plankton can be observed using the
foldscope. Simple slides of the settled biomass can by created using the
provided foldscope paper or film slides.
#IndiaFoldscopePhase1
– DBT Foldscope Category B Project Details:
Project
Title: Integrating microscopy and satellite oceanography to understand the
dynamics of planktonic communities around the Andaman Islands
Principal
Investigator: Mahima Jaini, Marine Research Officer, Dakshin Foundation
Location:
Andaman Nicobar Environment Team, Wandoor, South Andaman, India
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