Observation of stomata of onion peel and bulb

Dec 04, 2018 • 8:15 AM UTC

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140x Magnification

Microorganisms

Saisha

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The epidermal cells of an onion act as a protective barrier against the microbes. A layer of the epidermal cells were peeled from an onion bulb and leaf was examined as follows: The inner transparent lining of an onion bulb was peeled. A small layer of the peeled onion was transferred to a watch glass containing the distilled water. Few drops of the safranin staining solution were then added to the watch glass and the onion peel was stained for 30 seconds. The stained onion peel was then transferred to another watch glass containing distilled water. The specimen was mounted with glycerine and attached to the microscopic slide with an adhesive tape. The specimen was then observed under the foldscope and then the foldscope containing the glass slide was placed on the stage of the compound microscope and was observed using the microscope’s 10 X objective lens.

Stomata on onion leaf under Foldscope

Stomata of onion bulb

Observation of stomata of onion peel and bulb

Saisha

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