Usually, the most creative (and difficult) process of live imaging is discovering how to grow/keep alive whatever you want to image under a glass slide. This is not trivial, since organisms sense local environment which has a dramatic effect on the sample.
In the latest design, we coupled a new way to mount live plant samples – by magnetically coupling leaves so you can image stomatal dynamics. Here is a quick tutorial video to do the same. Depending on what you are imaging, it’s helpful to have light on maximum intensity (without diffuser). If the image is not focused; play with the new ramp.
The real thrill of this is when you can setup time lapse; go to sleep and watch a leaf grow when you wake up in the morning. Just setting it up; will update tomorrow on the same.
#foldscope is an ultra light portable microscope. In the new design, we implemented a new feature to be able to do in-situ imaging where the microscope goes to places where things grow. No slides. No mounts. The microscope directly coupled (magnetically) to a living object and remains attached. We have been using this to image live stomatal dynamics. Here is a quick tutorial video – try this with your plants and share your results. No plants were harmed making this video. #croton #in-situImaging #foldscope #fieldscience #stomata #plantcells
Just some quick observations. I find it remarkable how much the cellular geometry changes with every few millimeters.