S SHARMILA

College – Vellalar College for Women (Autonomous)


 Prinicipal Investigator – Dr. S. Sharmila


 Project Assistants – E.K. Ramya and S. Mownika


 The PG and Research Department of Botany organized a Guest lecture Programme on the topic entitled “Applications of Foldscope in Bioscience and Ecosystem Biomonitoring through Environmental DNA Metaborcoding approach” on 10.01.2019 under DBT –Foldscope Project Scheme. Dr. G. Gurusubramanian, Principal Investigator, DBT – Foldscope Scheme Project, North East Collaborator, Professor and Head of Zoology, Mizoram University, Aizawl, served as a resource person and delivered a brainstroming lecture to all the UG, PG and Research Scholars of the Botany Department.  He introduced the Foldscope to the student community and also explained about the Environmental DNA (eDNA), Community DNA (cDNA) and Next Generation Sequencing (NGS).  He emphasized some of the DNA databases such as EMBL, GenBank, DDBJ, Barcoding and MetaBarcoding approaches. At the end of the lecture he suggested some job oriented opportunities and its current scenario.

<figure class="wp-block-image">
 <img alt="1" class="wp-image-120406 gcloudimg" data-attachment-id="120406" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1547124148","copyright":"","focal_length":"3.57","iso":"640","shutter_speed":"0.05","title":"","orientation":"0"}' data-image-title="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120404/1-21.jpg"/>
 <figcaption>
 
 Dr. G. Gurusubramanian, Principal Investigator, DBT – Foldscope Project Scheme
 
 Mizoram University
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="2" class="wp-image-120407 gcloudimg" data-attachment-id="120407" data-comments-opened="1" data-image-meta='{"aperture":"2.2","credit":"","camera":"LND-AL30","caption":"","created_timestamp":"1547118888","copyright":"","focal_length":"3.462","iso":"996","shutter_speed":"0.068480912","title":"","orientation":"0"}' data-image-title="2" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120404/2-22.jpg"/>
 <figcaption>
 Introducing &amp; Summarizing Foldscope tool to the Students
 
 
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="3" class="wp-image-120411 gcloudimg" data-attachment-id="120411" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1547118065","copyright":"","focal_length":"3.57","iso":"868","shutter_speed":"0.05","title":"","orientation":"0"}' data-image-title="3" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120404/3-23.jpg"/>
 <figcaption>
 Describing the Foldscope Sheet
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="4" class="wp-image-120412 gcloudimg" data-attachment-id="120412" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1547117954","copyright":"","focal_length":"3.57","iso":"531","shutter_speed":"0.04","title":"","orientation":"0"}' data-image-title="4" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120404/4-16.jpg"/>
 <figcaption>
 Explaining the Application &amp; Usage of Foldscope
 </figcaption>
</figure>
<figure class="wp-block-image">
 <img alt="5" class="wp-image-120413 gcloudimg" data-attachment-id="120413" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1547123303","copyright":"","focal_length":"3.57","iso":"640","shutter_speed":"0.058823529411765","title":"","orientation":"0"}' data-image-title="5" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_120404/5-12.jpg"/>
 <figcaption>
 At the end of the session speaker clarifying the doubt of student
 </figcaption>
</figure>

WORKSHOP ON FOLDSCOPE-APPLICATIONS OF FOLDSCOPE IN BIOSCIENCE

College – Vellalar College for Women (Autonomous)


 Prinicipal Investigator – Dr. S. Sharmila


 Project
Assistants – E.K. Ramya and S. Mownika


 Foldscope is designed to bring paper microscopy out of science laboratories and into the hands of people around the world.  The Research Committee of Vellalar College for Women (Autonomous), Erode has organized a UGC funded State level Workshop on the topic entitled
 
 “Tools to Revitalize Research and Teaching”
 
 on 28.02.2019. Dr. S. Sharmila, Principal Investigator, DBT – Foldscope Project Scheme, Assistant Professor in Botany, Vellalar College for Women (Autonomous), Erode-12, served as a resource person and delivered a motivative lecture of Foldscope to all the Staff members and Research Scholars of our institution. She introduces the Foldscope to our own faculty members of various Departments and explained about ultra-affordable, paper microscope and it give optical quality similar to conventional research microscopes. She emphasized the assembly and handling with their importance and usage of Foldscope. The participants were very excited on hearing the description of Foldscope by the trainer. At end of the session, they clarify the doubts about foldscope.


 Dr.
S. Sharmila, Principal Investigator introduced the Foldscope to Research
Committee members

<figure class="wp-block-image">
 <img alt="1" class="wp-image-135988 gcloudimg" data-attachment-id="135988" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1551355850","copyright":"","focal_length":"3.57","iso":"640","shutter_speed":"0.05","title":"","orientation":"0"}' data-image-title="1" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_135987/1-22.jpg"/>
 <figcaption>
 
 </figcaption>
</figure>

 She talk about the differentiation among microscope and foldscope

<figure class="wp-block-image">
 <img alt="2" class="wp-image-135989 gcloudimg" data-attachment-id="135989" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1551356205","copyright":"","focal_length":"3.57","iso":"125","shutter_speed":"0.03030303030303","title":"","orientation":"0"}' data-image-title="2" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_135987/2-22.jpg"/>
</figure>

 Explained
an assembly of foldscope components and tools

<figure class="wp-block-image">
 <img alt="3" class="wp-image-135990 gcloudimg" data-attachment-id="135990" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="3" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_135987/3-21.jpg"/>
</figure>

WORKSHOP ON FOLDSCOPE- “TOOLS TO REVITALIZE RESEARCH AND TEACHING”

Name of the Institution – Vellalar College for Women (Autonomous), Erode-12


 Prinicipal Investigator – Dr. S. Sharmila, Assistant Professor in
Botany


 Project Assistants – Mrs. E.K. Ramya and Ms. S. Mownika


 

<div class="wp-block-image">
 <figure class="aligncenter is-resized">
 <img alt="IMG_4313" class="wp-image-161806 gcloudimg" data-attachment-id="161806" data-comments-opened="1" data-image-meta='{"aperture":"3.2","credit":"","camera":"Canon IXUS 155","caption":"","created_timestamp":"1561207594","copyright":"","focal_length":"4.766","iso":"200","shutter_speed":"0.0008","title":"","orientation":"1"}' data-image-title="IMG_4313" height="314" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_161805/IMG_4313.jpg" width="419"/>
 <figcaption>
 
 
 5
 
 th
 
 Standard boy viewing specimens – Foldscope
 
 </figcaption>
 </figure>
</div>
<div class="wp-block-image">
 <figure class="aligncenter is-resized">
 <img alt="IMG_4311" class="wp-image-161807 gcloudimg" data-attachment-id="161807" data-comments-opened="1" data-image-meta='{"aperture":"3.2","credit":"","camera":"Canon IXUS 155","caption":"","created_timestamp":"1561207560","copyright":"","focal_length":"4.766","iso":"200","shutter_speed":"0.002","title":"","orientation":"1"}' data-image-title="IMG_4311" height="332" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_161805/IMG_4311.jpg" width="443"/>
 <figcaption>
 
 
 Foldscope in Tribal Women’s hand
 
 </figcaption>
 </figure>
</div>

 My study site is an evergreen forest with rich vegetation of floras and faunas. It is located in the Western Ghats of Coimbatore District, Tamil Nadu. Geographically, the entire area of Manar village is lies between 11°29’- 11°48’N latitude and 70°50’- 77°27’E longitude. The knowledge of ethno history of the Irulas tribal group is very essential for a good ethnobotanical fieldwork. Totally six villages are occurred in my study area. Among the total of six villages, I have explore the invention of foldscope to Veerakkal village people. The most of the people in Veerakkal village are uneducated, so they have wondered to see and handle the foldscope with eagerness. The total number of people were 42 containing 14 families in that 21 males followed by female 21. Their major income is generated from  minor products like honey, vegetables, fruits and also sale in the local market and tribal co-operative societies. They planted many fruit trees like mango, banana and jack fruit around their houses. I hope that foldscope will surely helpful and motivational tool to them that brings educational awareness for tribal youngsters.

INTRODUCING FOLDSCOPE TOOL TO TRIBAL COMMUNITY  (IRULAS) – MANAR BEAT, KARAMADAI, COIMBATORE DISTRICT

Foldscope is so affordable and can easy to used anywhere, it brings science to our daily life activities. I’m looking forward to work with the foldscope it is very easy to carry and handling a foldsope is innovative learning to me.


 With the help of foldscope I observed anatomical characters in plant stem and also studied the trichome morphology. This experience was amazing to me.

<h3>
 
 Anatomy – 1
 
</h3>

 
 
 Acalypha indica
 
 
 Linn.


 
 
 
 
 My first anatomical section was taken from
 
 Acalypha indica
 
 Linn. commonly called as Indian copperleaf belongs to the family Euphorbiaceae


 Habit of Acalypha indica


 <img alt="Habit of Acalypha indica" class="aligncenter wp-image-39101 size-medium gcloudimg" data-attachment-id="39101" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527772867","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0060975609756098","title":"","orientation":"0"}' data-image-title="Habit of Acalypha indica" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/IMG_20180531_132107.jpg" width="225"/>


 Here is the first foldscope image….



<figure aria-describedby="caption-attachment-39110" class="wp-caption aligncenter" id="attachment_39110" style="width: 232px">
 <img alt="T.S of Acalypha indica stem" class="wp-image-39110 size-medium gcloudimg" data-attachment-id="39110" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527680963","copyright":"","focal_length":"3.57","iso":"108","shutter_speed":"0.01","title":"","orientation":"1"}' data-image-title="T.S of Acalypha indica stem" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/T.S-of-Acalypha-indica-stem.jpg" width="232"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39110">
 T.S of Acalypha indica stem
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-39114" class="wp-caption aligncenter" id="attachment_39114" style="width: 256px">
 <img alt="Acalypha trichome" class="wp-image-39114 size-medium gcloudimg" data-attachment-id="39114" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527681433","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0039370078740157","title":"","orientation":"1"}' data-image-title="Acalypha trichome" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/Acalypha-trichome.jpg" width="256"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39114">
 Beautiful view of Unicellular trichome
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-39119" class="wp-caption aligncenter" id="attachment_39119" style="width: 247px">
 <img alt="vascular bundle" class="size-medium wp-image-39119 gcloudimg" data-attachment-id="39119" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527680737","copyright":"","focal_length":"3.57","iso":"119","shutter_speed":"0.02","title":"","orientation":"1"}' data-image-title="vascular bundle" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/vascular-bundle.jpg" width="247"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39119">
 View of Vascular bundles
 </figcaption>
</figure>

 
 Important characters:
 

<ul>
 <li>
 Presence of thick cuticle and non-glandular unicellular trichomes in epidermal region.
 </li>
 <li>
 Followed by 2-3 layers of collenchyma and rest are parenchymatous cortex.
 </li>
 <li>
 With the help of foldscope I have clearly observed the continuous ring of vascular tissues made up of xylem and phloem.
 </li>
</ul>

 With the support of foldscope under high magnification I clearly viewed Sphaeraphides and glandular matter in cortex and pith region.

<h3 style="text-align: left">
 
 Anatomy – 2
 
</h3>

 
 
 Adhatoda vasica
 
 
 
 Nees
 
 .
 
 

<figure aria-describedby="caption-attachment-39123" class="wp-caption aligncenter" id="attachment_39123" style="width: 225px">
 <img alt="Adhatoda vasica habit" class="size-medium wp-image-39123 gcloudimg" data-attachment-id="39123" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527773041","copyright":"","focal_length":"3.57","iso":"105","shutter_speed":"0.01","title":"","orientation":"0"}' data-image-title="Adhatoda vasica habit" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/Adhatoda-vasica-habit.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39123">
 Habit of Adhatoda vasica Nees
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-39124" class="wp-caption aligncenter" id="attachment_39124" style="width: 225px">
 <img alt="side view of A.vasica" class="size-medium wp-image-39124 gcloudimg" data-attachment-id="39124" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527773131","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="side view of A.vasica" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/side-view-of-A.vasica.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39124">
 I went side to the tree and got a good look at that thin low branches that hold flowers
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-39125" class="wp-caption aligncenter" id="attachment_39125" style="width: 225px">
 <img alt="blooming twig of A.v" class="size-medium wp-image-39125 gcloudimg" data-attachment-id="39125" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527773153","copyright":"","focal_length":"3.57","iso":"115","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="blooming twig of A.v" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/blooming-twig-of-A.v.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39125">
 Blooming twig
 </figcaption>
</figure>

 Adhatoda vasica commonly called as Malabar nut belongs to the family Acanthaceae.

<figure aria-describedby="caption-attachment-39126" class="wp-caption aligncenter" id="attachment_39126" style="width: 225px">
 <img alt="T.S of A.v" class="size-medium wp-image-39126 gcloudimg" data-attachment-id="39126" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527682607","copyright":"","focal_length":"3.57","iso":"168","shutter_speed":"0.01","title":"","orientation":"1"}' data-image-title="T.S of A.v" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/T.S-of-A.v.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39126">
 T.S of Adhatoda vasica stem – Foldscope image
 </figcaption>
</figure>

 <img alt="IMG_20180530_120453" class="aligncenter size-medium wp-image-39127 gcloudimg" data-attachment-id="39127" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527681893","copyright":"","focal_length":"3.57","iso":"113","shutter_speed":"0.03030303030303","title":"","orientation":"1"}' data-image-title="IMG_20180530_120453" data-recalc-dims="1" height="219" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/IMG_20180530_120453.jpg" width="300"/>


 
 Important characters:
 

<ul>
 <li>
 I adjusted focus ramp in foldscope I clearly seen the vascular bundle they are arranged in siphonostele pattern which encloses a wide central pith.
 </li>
 <li>
 Starch grains and calcium oxalate crystals are seen in the middle region under high zoom foldscope image.
 </li>
</ul>

 
 
 Euphorbia hirta
 
 
 L.

<figure aria-describedby="caption-attachment-39130" class="wp-caption aligncenter" id="attachment_39130" style="width: 225px">
 <img alt="Euphorbia hirta habit" class="size-medium wp-image-39130 gcloudimg" data-attachment-id="39130" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527772953","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0086206896551724","title":"","orientation":"0"}' data-image-title="Euphorbia hirta habit" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/Euphorbia-hirta-habit.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39130">
 Aerial view of Euphorbia hirta
 </figcaption>
</figure>

 
 Trichomes:
 

<figure aria-describedby="caption-attachment-39133" class="wp-caption aligncenter" id="attachment_39133" style="width: 300px">
 <img alt="trichome E.h" class="size-medium wp-image-39133 gcloudimg" data-attachment-id="39133" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1527686241","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0084033613445378","title":"","orientation":"1"}' data-image-title="trichome E.h" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39100/trichome-E.h.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39133">
 This is my eminent pic in foldscope I have never taken before – Trichome
 </figcaption>
</figure>

 I really surprised to view the single trichome in the outer region which is multicellular and uniseriate.


 At that time I awe to capture that adorable pic in my mobile phone with the help of foldscope.


 So I greatfully acknowledge Dr. Manu prakash Ms. Christine Q Kurinara, and the prakash lab at Stanford university for encouraging and supporting me in this project.


 I also thank department of biotechnology for providing me the wonderful opportunity to exposure myself in foldscope.

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MORPHOANATOMICAL CHARACTERS OF MEDICINAL PLANTS  IN MANNAR REGION

Mannar hill in Karamadai range is one of the most important geographic regions in Western Ghats with rich biodiversity.


 To understand the eco-system in reserved forest we choose Mannar hill as our study area and applied Litter fall, litter accumulation and litter decomposition analysis.


 
 Study area
 
 :


 Mannar hill range view located in Western ghats, Coimbatore, Tamil Nadu. It’s wonderful hill station which is filled with medical treasury  and it is located near Baralikkadu.

<figure aria-describedby="caption-attachment-39154" class="wp-caption aligncenter" id="attachment_39154" style="width: 300px">
 <img alt="" class="wp-image-39154 size-medium gcloudimg" data-attachment-id="39154" data-comments-opened="1" data-image-meta='{"aperture":"5.7","credit":"","camera":"DSC-W320","caption":"","created_timestamp":"1267353136","copyright":"","focal_length":"18.8","iso":"80","shutter_speed":"0.004","title":"","orientation":"1"}' data-image-title="" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39115/DSC08807.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39154">
 Hill view of Mannar hill range
 </figcaption>
</figure>

 My study site is a evergreen forest with rich vegetation of floras and faunas. In this area microorganisms play a major role for decomposition.


 In my first visit into the forest I’m really surprised to see the faunas like Honeybees, Ant, Butterflies, Monkeys, Earthworm, Millipedes etc.,


 At that time I became more enthusiastic to capture that bewitching pic in my mobile phone with the help of foldscope.


 For litter dynamics calculation I have planned to study the followings:

<ol>
 <li>
 Litter fall
 </li>
 <li>
 Litter accumulation
 </li>
 <li>
 Litter decomposition
 </li>
</ol>

 
 Litterfall
 

<ul>
 <li>
 Based on the surved list I have choosen two dominant species and two associated species in mannar hill ranges.
 </li>
 <li>
 To analysis the litterfall of selected species, Trap had been placed in trees for seasonal studies.
 </li>
 <li>
 For litter fall analysis trap was fixed in size of 1feet × 1.5 feet upon the dominant trees.
 </li>
</ul>
<figure aria-describedby="caption-attachment-39159" class="wp-caption aligncenter" id="attachment_39159" style="width: 225px">
 <img alt="IMG-20180531-WA0007" class="wp-image-39159 size-medium gcloudimg" data-attachment-id="39159" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="IMG-20180531-WA0007" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39115/IMG-20180531-WA0007.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39159">
 This is my litter fall trap
 </figcaption>
</figure>

 
 Litter Accumulation
 

<ul>
 <li>
 To analysis the litter accumulation of selected species, Trap had been placed under the tree. I constructed 1m x 1m size of meter quadrat.
 </li>
 <li>
 I focused mainly litter accumulation in meter quadrat.
 </li>
</ul>
<figure aria-describedby="caption-attachment-39158" class="wp-caption aligncenter" id="attachment_39158" style="width: 225px">
 <img alt="IMG_20180520_133358" class="wp-image-39158 size-medium gcloudimg" data-attachment-id="39158" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1526823238","copyright":"","focal_length":"3.57","iso":"103","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="IMG_20180520_133358" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39115/IMG_20180520_133358.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39158">
 This is my quadrat picture with high litter accumulation
 </figcaption>
</figure>

 
 Litter decomposition
 

<ul>
 <li>
 This is an interesting part in litter dynamics. For this study, I brought nets in the size of 20cm x 10cm for decomposition of litter with the help of microbes.
 </li>
 <li>
 For litter decomposition studies, I placed neted litter bags into the soil holding leaves, twigs and miscellenious parts.
 </li>
</ul>
<figure aria-describedby="caption-attachment-39161" class="wp-caption aligncenter" id="attachment_39161" style="width: 300px">
 <img alt="IMG-20180601-WA0004" class="wp-image-39161 size-medium gcloudimg" data-attachment-id="39161" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="IMG-20180601-WA0004" data-recalc-dims="1" height="225" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_39115/IMG-20180601-WA0004.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-39161">
 This is my net litter bag
 </figcaption>
</figure>

 As per the schedule of work plan, I have started my work in the middle of  May 2018. In this regard, I have planned all the necessary steps to be followed for litter fall, litter accumulation and litter decomposition.


 I will planned to capture the microorganisms that is used for decomposition in the month of June 2018 with the help of foldscope magnification.


 I am eagerly waiting to study the microorganisms because I knowtFhefoldscope is so affordable, handy and easy to use.


 “Awaiting for next step follow up”


 I am sure that, I will identify the different type of decomposing microorganisms in the next month with the help of foldscope. I am coincide this foldscope will enrich my carrier and develop my project work.



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LITTER STUDY IN MANNAR HILL RANGE

ONE DAY DBT FUNDED SKILL DEVELOPMENT WORKSHOP ON FOLDSCOPE ASSEMBLY  AND IT’S USAGE

ANATOMY:
 

<ul>
 <li>
 
 Tridax procumbens
 
 belongs to the family Asteraceae.
 </li>
 <li>
 In India it is used for Wound healing, Anticoagulant, Antifungal and act as insect repellent.
 </li>
</ul>
<figure aria-describedby="caption-attachment-43530" class="wp-caption aligncenter" id="attachment_43530" style="width: 331px">
 <img alt="Untitled" class="wp-image-43530 gcloudimg" data-attachment-id="43530" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"1"}' data-image-title="Untitled" data-recalc-dims="1" height="248" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/Untitled-2.jpg" width="331"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43530">
 T.S of Tridax procumbens stem
 </figcaption>
</figure>

 
 TRICHOME:
 


 
 Important characters:
 

<ul>
 <li>
 
 Tridax procumbens
 
 consisted of glandular and non – glandular type of trichomes.
 </li>
</ul>
<figure aria-describedby="caption-attachment-43535" class="wp-caption aligncenter" id="attachment_43535" style="width: 300px">
 <img alt="IMG-20180623-WA0035" class="size-medium wp-image-43535 gcloudimg" data-attachment-id="43535" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1529742326","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"1"}' data-image-title="IMG-20180623-WA0035" data-recalc-dims="1" height="266" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/IMG-20180623-WA0035.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43535">
 Simple multicellular hair in Tridax procumbens L.
 </figcaption>
</figure>

 
 HISTOCHEMICAL ANALYSIS:
 

<figure aria-describedby="caption-attachment-43536" class="wp-caption aligncenter" id="attachment_43536" style="width: 272px">
 <img alt="IMG-20180623-WA0041" class="size-medium wp-image-43536 gcloudimg" data-attachment-id="43536" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1529742326","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"1"}' data-image-title="IMG-20180623-WA0041" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/IMG-20180623-WA0041.jpg" width="272"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43536">
 Carbohydrate – Barfoed’s reagent (It appears pink color) followed by Kokate et al. (1995)
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-43537" class="wp-caption aligncenter" id="attachment_43537" style="width: 300px">
 <img alt="IMG-20180623-WA0049" class="size-medium wp-image-43537 gcloudimg" data-attachment-id="43537" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1529742328","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"1"}' data-image-title="IMG-20180623-WA0049" data-recalc-dims="1" height="264" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/IMG-20180623-WA0049.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43537">
 Alkaloids – Wagner’s reagent (It appears brown color) followed by Jensen (1926)
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-43538" class="wp-caption aligncenter" id="attachment_43538" style="width: 300px">
 <img alt="IMG-20180623-WA0053" class="size-medium wp-image-43538 gcloudimg" data-attachment-id="43538" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1529742328","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"6"}' data-image-title="IMG-20180623-WA0053" data-recalc-dims="1" height="270" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/IMG-20180623-WA0053.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43538">
 Starch – Lugol’s iodine reagent (It appears blue color) followed by Jensen (1926)
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-43539" class="wp-caption aligncenter" id="attachment_43539" style="width: 262px">
 <img alt="IMG-20180623-WA0063" class="wp-image-43539 gcloudimg" data-attachment-id="43539" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"1529742330","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"1"}' data-image-title="IMG-20180623-WA0063" data-recalc-dims="1" height="337" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_43523/IMG-20180623-WA0063.jpg" width="262"/>
 <figcaption class="wp-caption-text" id="caption-attachment-43539">
 The picture shows the embryo of Tridax procumbens L. – Globular stage
 </figcaption>
</figure>

PHARMACOGNOSTICAL CHARACTERIZATION OF TRIDAX PROCUMBENS L.    MEDICINAL HERB IN – MANNAR REGION

College – Vellalar College for Women (Autonomous), Erode.


 Principal Investigator – Dr. S. Sharmila


 Project Assistant – E.K. Ramya, S.Mownika.


 With the help of foldscope I have observed the anatomical characters of
 
 Rauvolfia serpentina
 
 and
 
 Acacia caesia
 
 stem and also studied the trichome morphology.


 
 Anatomy – 1
 


 
 
 Rauvolfia serpentina
 
 
 
 (L.) Benth.
 


 
 Rauvolfia serpentina
 
 commonly called as snakewood belongs to the family Apocynaceae.

<figure aria-describedby="caption-attachment-62024" class="wp-caption aligncenter" id="attachment_62024" style="width: 225px">
 <img alt="IMG_20180905_122130" class="wp-image-62024 size-medium gcloudimg" data-attachment-id="62024" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1536150090","copyright":"","focal_length":"3.57","iso":"134","shutter_speed":"0.02","title":"","orientation":"0"}' data-image-title="IMG_20180905_122130" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/IMG_20180905_122130.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-62024">
 Beautiful flower blooms in Undershrub habitat
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-62025" class="wp-caption aligncenter" id="attachment_62025" style="width: 225px">
 <img alt="IMG_20180905_122536" class="size-medium wp-image-62025 gcloudimg" data-attachment-id="62025" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1536150336","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.0024691358024691","title":"","orientation":"0"}' data-image-title="IMG_20180905_122536" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/IMG_20180905_122536.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-62025">
 Fruit of Rauvolfia serpentina
 </figcaption>
</figure>
<figure aria-describedby="caption-attachment-62026" class="wp-caption aligncenter" id="attachment_62026" style="width: 208px">
 <img alt="close view of trichome" class="size-medium wp-image-62026 gcloudimg" data-attachment-id="62026" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1536218081","copyright":"","focal_length":"3.57","iso":"100","shutter_speed":"0.02","title":"","orientation":"1"}' data-image-title="close view of trichome" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/close-view-of-trichome.jpg" width="208"/>
 <figcaption class="wp-caption-text" id="caption-attachment-62026">
 Trichome of
 
 Rauvolfia serpentina
 
 in stem – Close view
 </figcaption>
</figure>

 <img alt="Raw. stem" class="size-medium wp-image-62028 aligncenter gcloudimg" data-attachment-id="62028" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1536217628","copyright":"","focal_length":"3.57","iso":"140","shutter_speed":"0.04","title":"","orientation":"1"}' data-image-title="Raw. stem" data-recalc-dims="1" height="258" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/Raw.-stem.jpg" width="300"/>

<figure aria-describedby="caption-attachment-62029" class="wp-caption aligncenter" id="attachment_62029" style="width: 298px">
 <img alt="Raw. vascular bundle" class="size-medium wp-image-62029 gcloudimg" data-attachment-id="62029" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note3","caption":"","created_timestamp":"1536217946","copyright":"","focal_length":"3.57","iso":"232","shutter_speed":"0.04","title":"","orientation":"1"}' data-image-title="Raw. vascular bundle" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/Raw.-vascular-bundle.jpg" width="298"/>
 <figcaption class="wp-caption-text" id="caption-attachment-62029">
 T.S of
 
 Rauvolfia serpentina stem
 
 –Foldscope image
 </figcaption>
</figure>

 
 Important characters:
 

<ul>
 <li>
 Indian snakeroot has been used in traditional Ayurvedic medicine in India, mainly for high blood pressure and mental illness.
 </li>
</ul>

 
 Anatomy – 2
 


 
 
 Acacia caesia
 
 
 
 (L.) Willd.
 


 
 Acacia caesia
 
 belongs to the family Mimosaceae and it is commonly called as Black catechu.

<figure aria-describedby="caption-attachment-62054" class="wp-caption aligncenter" id="attachment_62054" style="width: 300px">
 <img alt="Acacia stem" class="size-medium wp-image-62054 gcloudimg" data-attachment-id="62054" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="Acacia stem" data-recalc-dims="1" height="272" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_62022/Acacia-stem.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-62054">
 T.S of Acacia caesia stem
 </figcaption>
</figure>

 
 Important characters:
 

<ul>
 <li>
 The anatomical characters was observed using Foldscope research tool.
 </li>
 <li>
 The Foldscope studies revealed the presence of uniseriate glandular hairs, fibres, chambered crystals, vasicentric vessels and fusiform parenchyma cells.
 </li>
</ul>

RAUVOLFIA SERPENTINA AND ACACIA CAESIA &#8211; ANATOMICAL  VIEW UNDER FOLDSCOPE

Dr. S. Sharmila, Principal Investigator, Assistant Professor in Botany, Vellalar College for Women, Thindal, Erode-12, Tamilnadu have organized a Northeast collaborative foldscope workshop on the topic
 
 “Foldscope – A new research tool for better understanding of Biological Sciences”
 
 under
 
 DBT – microgrant Foldscope Project
 
 funded by
 
 Department of Biotechnology,
 
 Ministry of Science and Technology, Govt. of India in collaboration with Foldscope Instruments Inc. and Prakash Lab (Stanford), USA on 13
 
 th
 
 November 2018.


 The workshop was conducted in Mizoram-University, which is a central university under University Grants Commission, Government of India, and the university was established on 2 July 2001, by the Mizoram University Act (2000) by the Parliament of India. According to the Act, the objectives of the university are  to take appropriate measures for promoting innovations in teaching-disciplinary studies and research; to educate and train man-power in development of the state of Mizoram; and to pay special attention to the improvement of social and economic conditions and welfare of people in that State, their intellectual, academic and cultural development”. Keeping these objectives in view, Mizoram University has embarked on various programmes for academic and administrative development.


 The 12
 
 th
 
 Annual Convention of ABAP &amp; International Conference on Biodiversity, Environment and Human Health: Innovations and Emerging Trends (BEHIET – 2018) was organized on November 12 – 14
 
 th
 
 2018 by the Departments (Botany, Zoology &amp; Biotechnology) of Mizoram University, Aizawl. At this junction, I have presented my research findings entitled “Anatomical exploration under foldscope and phytopharmacological studies of
 
 Acacia caesia
 
 (L.) Willd”.


 <figure aria-describedby="caption-attachment-86429" class="wp-caption aligncenter" id="attachment_86429" style="width: 300px">
 <img alt="2" class="wp-image-86429 size-medium gcloudimg" data-attachment-id="86429" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="2" data-recalc-dims="1" height="169" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_86427/2-15.jpg" width="300"/>
 <figcaption class="wp-caption-text" id="caption-attachment-86429">
 Dr. S. Sharmila, Principal investigator explained about the Theme of the Workshop
 </figcaption>
 </figure>


 <figure aria-describedby="caption-attachment-86431" class="wp-caption aligncenter" id="attachment_86431" style="width: 225px">
 <img alt="1" class="wp-image-86431 size-medium gcloudimg" data-attachment-id="86431" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1542126935","copyright":"","focal_length":"3.57","iso":"320","shutter_speed":"0.03030303030303","title":"","orientation":"0"}' data-image-title="1" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_86427/1-18.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-86431">
 Presenting my Research work – Foldscope
 </figcaption>
 </figure>


 Foldscopes are origami microscopes that have potential to change the way we view life and microorganisms around us. For popularizing the origami microscope, I have conducted a Foldscope workshop in the Conference and the workshop was attended by the participants who came from various states and the same central University life science students who came for attending the conference.  In view of spreading knowledge and awareness about foldscope, the same were explained by putting stall during the conference where assemble methods and live sample was viewed using mobile camera. Initially the session was beginned with power point presentation for easy understanding and also you-tube videos were explained on ‘How to assemble the foldscope’. Foldscope was totally new to them. They never heard it before about foldscope. They assembled the pieces and tried to peep through it and become well – versed with its usage. They become very much interested to assemble it. At the same day memorandum of understanding was signed between Vellalar College for Women, Erode and Mizoram University, Aizawl for collaborative research work.


 <figure aria-describedby="caption-attachment-86432" class="wp-caption aligncenter" id="attachment_86432" style="width: 225px">
 <img alt="3" class="size-medium wp-image-86432 gcloudimg" data-attachment-id="86432" data-comments-opened="1" data-image-meta='{"aperture":"2","credit":"","camera":"Redmi Note 4","caption":"","created_timestamp":"1542102366","copyright":"","focal_length":"3.57","iso":"800","shutter_speed":"0.071428571428571","title":"","orientation":"0"}' data-image-title="3" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_86427/3-15.jpg" width="225"/>
 <figcaption class="wp-caption-text" id="caption-attachment-86432">
 Participants viewing their samples in live manner
 </figcaption>
 </figure>


 <figure aria-describedby="caption-attachment-86433" class="wp-caption aligncenter" id="attachment_86433" style="width: 169px">
 <img alt="4" class="size-medium wp-image-86433 gcloudimg" data-attachment-id="86433" data-comments-opened="1" data-image-meta='{"aperture":"0","credit":"","camera":"","caption":"","created_timestamp":"0","copyright":"","focal_length":"0","iso":"0","shutter_speed":"0","title":"","orientation":"0"}' data-image-title="4" data-recalc-dims="1" height="300" src="https://storage.googleapis.com/microcosmosdelta.appspot.com/images/transfer_86427/4-14.jpg" width="169"/>
 <figcaption class="wp-caption-text" id="caption-attachment-86433">
 Before the Stall
 </figcaption>
 </figure>

S SHARMILA

Earth

Posts

ONE DAY DBT FUNDED SKILL DEVELOPMENT WORKSHOP ON FOLDSCOPE ASSEMBLY AND IT’S USAGE

PHARMACOGNOSTICAL CHARACTERIZATION OF TRIDAX PROCUMBENS L. MEDICINAL HERB IN – MANNAR REGION

RAUVOLFIA SERPENTINA AND ACACIA CAESIA – ANATOMICAL VIEW UNDER FOLDSCOPE

NORTHEAST COLLABORATIVE TRAINING WORKSHOP ON FOLDSCOPE

WORKSHOP ON FOLDSCOPE-APPLICATIONS OF FOLDSCOPE IN BIOSCIENCE

WORKSHOP ON FOLDSCOPE- “TOOLS TO REVITALIZE RESEARCH AND TEACHING”

INTRODUCING FOLDSCOPE TOOL TO TRIBAL COMMUNITY (IRULAS) – MANAR BEAT, KARAMADAI, COIMBATORE DISTRICT

MORPHOANATOMICAL CHARACTERS OF MEDICINAL PLANTS IN MANNAR REGION

LITTER STUDY IN MANNAR HILL RANGE

ONE DAY DBT FUNDED SKILL DEVELOPMENT WORKSHOP ON FOLDSCOPE ASSEMBLY AND IT’S USAGE

PHARMACOGNOSTICAL CHARACTERIZATION OF TRIDAX PROCUMBENS L. MEDICINAL HERB IN – MANNAR REGION

RAUVOLFIA SERPENTINA AND ACACIA CAESIA – ANATOMICAL VIEW UNDER FOLDSCOPE

NORTHEAST COLLABORATIVE TRAINING WORKSHOP ON FOLDSCOPE

WORKSHOP ON FOLDSCOPE-APPLICATIONS OF FOLDSCOPE IN BIOSCIENCE

WORKSHOP ON FOLDSCOPE- “TOOLS TO REVITALIZE RESEARCH AND TEACHING”

INTRODUCING FOLDSCOPE TOOL TO TRIBAL COMMUNITY (IRULAS) – MANAR BEAT, KARAMADAI, COIMBATORE DISTRICT

MORPHOANATOMICAL CHARACTERS OF MEDICINAL PLANTS IN MANNAR REGION

LITTER STUDY IN MANNAR HILL RANGE

ONE DAY DBT FUNDED SKILL DEVELOPMENT WORKSHOP ON FOLDSCOPE ASSEMBLY AND IT’S USAGE

PHARMACOGNOSTICAL CHARACTERIZATION OF TRIDAX PROCUMBENS L. MEDICINAL HERB IN – MANNAR REGION

RAUVOLFIA SERPENTINA AND ACACIA CAESIA – ANATOMICAL VIEW UNDER FOLDSCOPE

NORTHEAST COLLABORATIVE TRAINING WORKSHOP ON FOLDSCOPE

Locations

Statistics

Type of Sample

Foldscope Lens Magnification