Day 7: Lichen cultures

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Continuing from my previous post, I am going to try and keep an eye on the succession of life in my lichen cultures. For this, I have 6 different lichens collected from different trees put into petridishes containing RO water. Below, I categorize all life I could see after 1 week.

Here are results from three of the plates. Dont miss out the third one. It is full of exciting life that I havent seen before.3plates

Plate-number Observation date Species observed
April-22-Plate1 April-29-2017  Long-ciliate, Colpoda, liitle Ciliate
April-22-Plate2 April-29-2017  Nematode, Large Bdelloid Rotifer, Tardigrade, Long ciliate, Ciliate-24621-2-LI, little ciliate
April-22-Plate3 April-29-2017  Nematode, Bdelloid Rotifer, Tardigrade, Amoeba, flagellate, Ciliate-24621-2-LI, flagellate, Ciliate with a terminal contractile vacuole, Colpoda
April-21-Plate1 April-29-2017  Nematode, Bdelloid Rotifer, Long ciliate, Stylonychia-like ciliate
April-21-Plate2 April-29-2017  Bdelloid Rotifer, Colpoda, Long ciliate
April-21-Plate3 April-29-2017  Nematode, Rotifer, Colpoda

1. April-22-Plate 1, Colpoda is the most common ciliate I have seen in over 20 lichen suspension cultures (see previous observation, this is a different species). It definitely is significantly associated with lichens. Its also very easy to grow. You can culture it in hay infusion. I have done it several times now.

2. April 22-Plate 2. This ciliate (Ciliate-24621-2-LI) is definitely a hypotrich ciliate (reminds me of Ciliate-21501-2-LI). I suspect it is related to Stylonychia. Would you be able to help identify it?.

3.  April22-Plate 3. It is amazing how much life is there in this plate…. I thought I’d put it in this post, but I’ll leave it in suspense until the next post 🙂 — To be continued.

4 Comments Add yours

  1. Manu Prakash says:

    What a wonderful reminder @Laks that things exist at places where we least expect them. What a wonderful and simple to keep/maintain collection.

    The back kick and back cirri of april22-plate2 ciliate is very characteristic.

    Cheers
    Manu

  2. Saad says:

    @laks – as always, great post. This is a great lesson for anyone else who is trying to keep detailed tracking of long-term cultures.

    You have a private zoo going 🙂
    Saad

  3. laksiyer says:

    Thanks @Manu and @Saad. There is so much to explore and this is one easy way to get surprised. Am going to move onto moss ecology next.

  4. Manu Prakash says:

    @Laks,

    You might also want to briefly glance to see what has been said in formal reports on lichen ecology. I am just so surprised; because I would not have expected this rich character. I think this one is worth following up scientifically!!

    Cheers
    Manu

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